Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, No. 1 Friendship Road, Yuzhong District, Chongqing 400016, China.
Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, No. 1 Friendship Road, Yuzhong District, Chongqing 400016, China.
Int Immunopharmacol. 2019 Jul;72:92-97. doi: 10.1016/j.intimp.2019.03.033. Epub 2019 Apr 8.
Clostridium difficile infection (CDI) is the primary cause of community- and health care-associated diarrhea. CXCL10, also known as IFN-γ-inducible protein of 10 (IP-10), is involved in various inflammatory diseases, but its role in CDI remains unknown. In this study, We determined the serum concentration of CXCL10 in 80 CDI patients and 76 sex & age-matched diarrhea patients by enzyme-linked immunosorbent assay (ELISA) and analyzed the correlation between CXCL10 levels and CDI disease severity parameters. Besides, we also measured the level of other cytokines and/or chemokines in CDI patients, such as IL-1β, IL-6, TNF-α and CXCL9. We found that serum CXCL10 in CDI patients was significantly higher compared with those in non-C. difficile diarrhea patients, especially in the moderate disease. Elevated serum CXCL10 correlated positively and significantly with severity score index (SSI) score in all CDI patients. CXCL10 levels were also positively correlated with WBC count, creatinine and inflammatory cytokines including, IL-1β, IL-6 and CXCL9, but negatively correlated with albumin. Furthermore, serum CXCL10 concentration could be significantly decreased after effective treatment of CDI. Therefore, the above results suggest that the up-regulated release of CXCL10 is important in the immunopathogenesis of CDI, and may be served as a potential alternative biomarker for the monitoring of CDI disease severity and therapeutic efficacy.
艰难梭菌感染（CDI）是社区和医疗保健相关腹泻的主要原因。趋化因子（C-X-C 基元）配体 10（CXCL10），也称为 IFN-γ 诱导蛋白 10（IP-10），参与各种炎症性疾病，但它在 CDI 中的作用尚不清楚。在这项研究中，我们通过酶联免疫吸附试验（ELISA）测定了 80 例 CDI 患者和 76 名性别和年龄匹配的腹泻患者的血清 CXCL10 浓度，并分析了 CXCL10 水平与 CDI 疾病严重程度参数之间的相关性。此外，我们还测量了 CDI 患者中其他细胞因子和/或趋化因子的水平，如 IL-1β、IL-6、TNF-α 和 CXCL9。我们发现，与非艰难梭菌腹泻患者相比，CDI 患者的血清 CXCL10 显著升高，尤其是在中度疾病中。在所有 CDI 患者中，升高的血清 CXCL10 与严重程度评分指数（SSI）评分呈正相关且显著相关。CXCL10 水平与白细胞计数、肌酐和包括 IL-1β、IL-6 和 CXCL9 在内的炎症细胞因子呈正相关，与白蛋白呈负相关。此外，CDI 有效治疗后，血清 CXCL10 浓度可显著降低。因此，上述结果表明，CXCL10 的上调释放在 CDI 的免疫发病机制中很重要，并且可能作为监测 CDI 疾病严重程度和治疗效果的潜在替代生物标志物。
