Department of Biological Sciences (Anatomy), Bauru School of Dentistry, University of São Paulo (USP), Bauru 17012-901, Brazil.
Department of Human Morphophysiology, Medical and Dentistry School, University of Marilia (UNIMAR), Marília 17525-902, Brazil.
Int J Mol Sci. 2019 Apr 10;20(7):1761. doi: 10.3390/ijms20071761.
Fibrin sealants derived from human blood can be used in tissue engineering to assist in the repair of bone defects. The objective of this study was to evaluate the support system formed by a xenograft fibrin sealant associated with photobiomodulation therapy of critical defects in rat calvaria. Thirty-six rats were divided into four groups: BC ( = 8), defect filled with blood clot; FSB ( = 10), filled with fibrin sealant and xenograft; BC ( = 8), blood clot and photobiomodulation; FSB ( = 10), fibrin sealant, xenograft, and photobiomodulation. The animals were killed after 14 and 42 days. In the histological and microtomographic analysis, new bone formation was observed in all groups, limited to the defect margins, and without complete wound closure. In the FSB group, bone formation increased between periods (4.3 ± 0.46 to 6.01 ± 0.32), yet with lower volume density when compared to the FSB (5.6 ± 0.45 to 10.64 ± 0.97) group. It was concluded that the support system formed by the xenograft fibrin sealant associated with the photobiomodulation therapy protocol had a positive effect on the bone repair process.
来源于人血的纤维蛋白粘合剂可用于组织工程，以协助修复骨缺损。本研究的目的是评估异种纤维蛋白粘合剂联合光生物调节疗法在大鼠颅骨临界缺损中的支撑系统。将 36 只大鼠分为四组：BC 组（n=8），用血凝块填充；FSB 组（n=10），用纤维蛋白粘合剂和异种移植物填充；BC 组（n=8），用血凝块和光生物调节疗法；FSB 组（n=10），用纤维蛋白粘合剂、异种移植物和光生物调节疗法。14 天和 42 天后处死动物。在组织学和微断层分析中，所有组均观察到新骨形成，仅限于缺损边缘，未完全闭合伤口。在 FSB 组中，骨形成在两个时期之间增加（4.3±0.46 至 6.01±0.32），但与 FSB 组（5.6±0.45 至 10.64±0.97）相比，体积密度较低。结论：与光生物调节治疗方案相关的异种纤维蛋白粘合剂支撑系统对骨修复过程有积极影响。
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