An Yin-Zhe, Heo Young-Ku, Lee Jung-Seok, Jung Ui-Won, Choi Seong-Ho
Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University, Seoul 03722, Korea.
Global Academy of Osseointegration, Neobiotech Co., Ltd., Seoul 06072, Korea.
Materials (Basel). 2017 Aug 10;10(8):927. doi: 10.3390/ma10080927.
In this study, the bone regeneration efficacy of dehydrothermally (DHT) cross-linked collagen membrane with or without a bone graft (BG) material was evaluated in a critical-sized rat model. An 8-mm-diameter defect was created in the calvaria of 40 rats, which were randomized into four groups: (1) control; (2) DHT; (3) BG; and, (4) DHT + BG. Evaluations were made at 2 and 8 weeks after surgery using micro-computed tomographic (micro-CT), histological, and histomorphometric analyses. Micro-CT analysis showed an increase in the new bone volume (NBV) of the BG and DHT + BG groups at 2 weeks after surgery, representing a significant difference ( < 0.05). At 8 weeks after surgery, the NBV increased in all four groups. However, larger NBVs were observed in the BG and DHT + BG groups, and a significant difference was no longer observed between the two groups. Histologic analysis demonstrated that the graft materials sustained the center of the defect in the BG and DHT + BG groups, which was shown in histomorphometric analysis as well. These results suggest that DHT membrane is a safe biomaterial with adequate tissue integration, and has a positive effect on new bone formation. Moreover, the best effects were achieved when DHT was used in conjunction with BG materials.
在本研究中，在临界大小的大鼠模型中评估了经脱氢热交联（DHT）的胶原膜在有或无骨移植（BG）材料情况下的骨再生效果。在40只大鼠的颅骨上制造一个直径8毫米的缺损，将其随机分为四组：（1）对照组；（2）DHT组；（3）BG组；以及（4）DHT + BG组。在术后2周和8周使用微型计算机断层扫描（micro-CT）、组织学和组织形态计量学分析进行评估。Micro-CT分析显示，术后2周时BG组和DHT + BG组的新骨体积（NBV）增加，差异有统计学意义（<0.05）。术后8周时，所有四组的NBV均增加。然而，BG组和DHT + BG组观察到更大的NBV，两组之间不再有显著差异。组织学分析表明，BG组和DHT + BG组的移植材料维持在缺损中心，组织形态计量学分析也显示了这一点。这些结果表明，DHT膜是一种具有充分组织整合性的安全生物材料，对新骨形成有积极作用。此外，DHT与BG材料联合使用时效果最佳。
