Division of Cancer Immunotherapy, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center, Kashiwa, Japan.
Second Department of Internal Medicine, Faculty of Medical Sciences, University of Fukui, Fukui, Japan.
Sci Rep. 2019 Apr 11;9(1):5925. doi: 10.1038/s41598-019-42392-7.
Biliary tract cancer (BTC) is an aggressive type of malignant tumour. Even after radical resection, the risk of recurrence is still high, resulting in a poor prognosis. Here, we investigated the usefulness of serum miRNAs as predictive markers of recurrence and prognosis for patients with BTC after radical surgery using 66 serum samples that were collected at three time points from 22 patients with BTC who underwent radical surgery. Using microarray analysis, we successfully identified six specific miRNAs (miR-1225-3p, miR-1234-3p, miR1260b, miR-1470, miR-6834-3p, and miR-6875-5p) associated with recurrence and prognosis of BTC after radical surgery. In addition, using a combination of these miRNAs, we developed a recurrence predictive index to predict recurrence in patients with BTC after operation with high accuracy. Patients having higher index scores (≥ cut-off) had significantly worse recurrence-free survival (RFS) and overall survival (OS) than those with lower index scores (<cut-off). Furthermore, the index was an independent factor related to RFS and OS by univariate and multivariate analyses using a Cox hazard proportional model. Overall, our results provided compelling evidence for the potential usefulness of specific serum miRNAs as effective predictive tools for recurrence and prognosis in patients with BTC who underwent radical surgery.
胆管癌（BTC）是一种侵袭性恶性肿瘤。即使进行根治性切除，复发的风险仍然很高，导致预后不良。在这里，我们使用从 22 名接受根治性手术的 BTC 患者在三个时间点收集的 66 份血清样本，研究了血清 miRNAs 作为预测 BTC 患者根治术后复发和预后的有用性。通过微阵列分析，我们成功鉴定出与 BTC 根治术后复发和预后相关的六种特定 miRNAs（miR-1225-3p、miR-1234-3p、miR1260b、miR-1470、miR-6834-3p 和 miR-6875-5p）。此外，我们使用这些 miRNAs 的组合，开发了一种复发预测指数，以高精度预测 BTC 患者术后的复发。指数得分较高（≥截止值）的患者的无复发生存率（RFS）和总生存率（OS）明显低于指数得分较低（<截止值）的患者。此外，该指数通过使用 Cox 风险比例模型的单因素和多因素分析是与 RFS 和 OS 相关的独立因素。总体而言，我们的研究结果为特定血清 miRNAs 作为 BTC 患者根治术后复发和预后的有效预测工具提供了有力的证据。
