1Department of Microbiology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran.
2Department of Microbiology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
Antimicrob Resist Infect Control. 2019 Mar 29;8:59. doi: 10.1186/s13756-019-0509-3. eCollection 2019.
The aim of this study was to characterize class 1,2 and 3 integrons in clinical MDR isolates in Kashan, Iran.
One hundred-eighty one were recovered from clinical specimens during November 2013 to October 2014. Antimicrobial susceptibility patterns were determined by disk diffusion method according to the Clinical and Laboratory Standards Institute (CLSI) guidelines for detection of MDR strains. Of the 181 146 (80.7%) of isolates were isolated from nosocomial infected patients and 150 (82.9%) identified as MDR isolates. The PCR amplification was used to show presence of class 1, 2 and 3 integrons among MDR strains. The PCR method and sequencing were used for evaluation of cassette content of integrons.
Of the MDR isolates, 150 (100%) and 55 (36.7%) carried and genes, respectively. None of the MDR isolates carried class 3 integrons. Amplification of conserved segment (CS) of class 1 and class 2 integrons revealed 10 different arrays including: No. cassette; , ; ; , ; , , ; , , ; , , , , , , , ; , , ; , , , , and 4 arrays including: No. cassette; ; ; , , respectively.
The finding of present study revealed a high prevalence of integrons especially class 1 among MDR isolates from nosocomial infections in Kashan, which led to rapid extension of MDR strains.
本研究旨在分析伊朗卡尚临床多重耐药（MDR）分离株中的 1 类、2 类和 3 类整合子。
从 2013 年 11 月至 2014 年 10 月采集的临床标本中分离出 181 株 MDR 株。采用纸片扩散法根据临床和实验室标准协会（CLSI）指南检测 MDR 菌株的药敏模式。181 株中，146 株（80.7%）分离自医院感染患者，150 株（82.9%）鉴定为 MDR 株。采用 PCR 扩增检测 MDR 株中 1 类、2 类和 3 类整合子的存在。PCR 方法和测序用于评估整合子的盒式元件内容。
在 MDR 株中，携带 基因的分别为 150 株（100%）和 55 株（36.7%）。MDR 株均未携带 3 类整合子。1 类和 2 类整合子保守区（CS）的扩增显示 10 种不同的排列，包括：无盒式元件； ， ； ， ； ， ； ， ； ， ； ， ； ， ； ， ， ， ， ， ， ， ， ；4 种排列，包括：无盒式元件； ， ， 。
本研究发现，卡尚医院感染的 MDR 分离株中，整合子特别是 1 类的流行率很高，导致 MDR 菌株迅速扩展。
