设计含马来酰亚胺的 S-S 键桥连 closo-十二硼烷（SSMID）：鉴定白蛋白上的独特修饰位点及研究细胞内摄取。
Design of S-S bond containing maleimide-conjugated closo-dodecaborate (SSMID): identification of unique modification sites on albumin and investigation of intracellular uptake.
机构信息
Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, R1-13, 4259, Nagatsuta-cho, Midori-ku, Yokohama, 226-8503, Japan.
出版信息
Org Biomol Chem. 2019 Jun 5;17(22):5496-5499. doi: 10.1039/c9ob00584f.
An S-S bond containing maleimide-conjugated closo-dodecaborate (SSMID) was synthesised for identification of albumin binding sites. Three Lys residues, Lys221, Lys413 and Lys431, were identified as SSMID modification sites in addition to Cys34 in bovin serum albumin (BSA). Fluorescent-labelled MID-BSA was found to be accumulated in the cytosol of HeLa cells.
一种含有马来酰亚胺修饰的闭式十二硼烷（SSMID）的 S-S 键被合成，用于鉴定白蛋白结合位点。除了牛血清白蛋白（BSA）中的半胱氨酸 34 外，还鉴定出三个赖氨酸残基，即赖氨酸 221、赖氨酸 413 和赖氨酸 431，为 SSMID 修饰位点。发现荧光标记的 MID-BSA 在 HeLa 细胞的细胞质中积累。
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