Li Yuanyuan, Zhang Xin, Chen Weijie, Liu Hongyang, Sun Liquan, Luo Aiqin
Beijing Institute of Technology, Beijing 100081, China.
Se Pu. 2019 Apr 8;37(4):392-397. doi: 10.3724/SP.J.1123.2018.10022.
In this work, a novel polymer membrane molecularly imprinted on a silanized glass substrate was prepared. Lysozyme was chosen as the template protein, and acrylamide and '-methylenebisacrylamide were used as the functional monomer and crosslinking agent, respectively. The adsorption equilibrium time, maximum adsorption capacity, specific recognition ability, reusability, and application of the prepared lysozyme-imprinted polymer membrane were investigated experimentally. The prepared polymer membrane exhibited special recognition ability and could quickly adsorb lysozyme. Under optimal conditions, the adsorption equilibrium time of the prepared polymer membrane for lysozyme was 5 min, and the adsorption behavior conformed to the Langmuir adsorption model; the theoretical maximum adsorption capacity was 42.5 mg/g, and the adsorption capacity for an egg sample was 30 mg/g. Additionally, the polymer membrane showed a high selectivity toward the template protein, lysozyme, and exhibited no obvious adsorption of several interfering proteins; the imprinting factor was 3.0. The maximum adsorption capacity of the polymer membrane decreased by 5% after being reused five times. The polymer membrane was used for the adsorption of lysozyme in samples, and good results were obtained. This strategy has potential for the separation and enrichment of target proteins in complex biological samples.
在本工作中，制备了一种分子印迹在硅烷化玻璃基底上的新型聚合物膜。选择溶菌酶作为模板蛋白，分别使用丙烯酰胺和N,N'-亚甲基双丙烯酰胺作为功能单体和交联剂。通过实验研究了所制备的溶菌酶印迹聚合物膜的吸附平衡时间、最大吸附容量、特异性识别能力、可重复使用性及应用。所制备的聚合物膜表现出特殊的识别能力，能够快速吸附溶菌酶。在最佳条件下，所制备的聚合物膜对溶菌酶的吸附平衡时间为5分钟，吸附行为符合朗缪尔吸附模型；理论最大吸附容量为42.5 mg/g，对鸡蛋样品的吸附容量为30 mg/g。此外，该聚合物膜对模板蛋白溶菌酶表现出高选择性，对几种干扰蛋白无明显吸附；印迹因子为3.0。聚合物膜重复使用五次后最大吸附容量下降了5%。该聚合物膜用于样品中溶菌酶的吸附，取得了良好的效果。该策略在复杂生物样品中目标蛋白的分离和富集方面具有潜力。
