Damasceno Jeziel D, Tosi Luiz R O, Santos Renato E R S, Mottram Jeremy C
The Wellcome Trust Centre for Molecular Parasitology, Institute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow, UK.
Department of Cell and Molecular Biology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.
Methods Mol Biol. 2019;1971:225-235. doi: 10.1007/978-1-4939-9210-2_11.
Induction of gene expression is a valuable approach for functional studies since it allows for the assessment of phenotypes without the need for clonal selection. Inducible expression can find a wide range of applications, from the study of essential genes to the characterization of overexpression of genes of interest. Here, we describe a detailed protocol for the use of the DiCre-based inducible gene expression system in Leishmania parasites. This is a tightly regulated induction system that allows for time- and dose-controlled expression of gene products, as rapidly as within 12 h.
基因表达的诱导是功能研究的一种有价值的方法，因为它无需进行克隆选择就能评估表型。诱导型表达具有广泛的应用，从必需基因的研究到感兴趣基因过表达的表征。在这里，我们描述了在利什曼原虫寄生虫中使用基于DiCre的诱导型基因表达系统的详细方案。这是一个严格调控的诱导系统，能够实现基因产物的时间和剂量控制表达，最快可在12小时内实现。
