Gross N, Bron C
Clin Exp Immunol. 1978 Aug;33(2):283-91.
An antiserum was obtained by immunization of rabbits with human thymocyte membrane fractions. After appropriate absorptions, the antiserum was shown to detect specifically a population of T-cells. When tested by complement-mediated cytotoxicity the antiserum lysed 95% of thymocytes, 65% of normal PBL and 45% of tonsillar lymphocytes. It was also cytotoxic for three different T-cell lines (MOLT-4, CCRF-CEM and CCRF-HSB-2). When peripheral or tonsillar lymphocytes were separated into populations enriched in B- and T-cells, the percentage of cells lysed by the antiserum correlated well with the proportion of E-rosetting cells. Treatment of PBL with the antiserum and complement resulted in an increase of SmIg-positive B-cells in the residual cell fraction, which could no longer form E-rosettes. Treatment of PBL with the antiserum alone completely inhibited the E-rosette formation. The cytotoxic index on PBL from patients with various lymphoid disorders always correlated with the proportion of T-cells as assessed by E-rosette formation. Finally, the absorptive capacity of thymocytes for the antiserum was ten times higher as compared to that of PBL or tonsil cells.
用人胸腺细胞膜组分免疫兔子获得了一种抗血清。经过适当吸收后，该抗血清被证明能特异性检测一群T细胞。通过补体介导的细胞毒性试验检测，该抗血清能裂解95%的胸腺细胞、65%的正常外周血淋巴细胞（PBL）和45%的扁桃体淋巴细胞。它对三种不同的T细胞系（MOLT - 4、CCRF - CEM和CCRF - HSB - 2）也具有细胞毒性。当外周血或扁桃体淋巴细胞被分离成富含B细胞和T细胞的群体时，被抗血清裂解的细胞百分比与E花环形成细胞的比例密切相关。用抗血清和补体处理PBL导致残余细胞部分中SmIg阳性B细胞增加，这些残余细胞不再能形成E花环。单独用抗血清处理PBL完全抑制了E花环的形成。各种淋巴疾病患者PBL的细胞毒性指数始终与通过E花环形成评估的T细胞比例相关。最后，胸腺细胞对抗血清的吸收能力比PBL或扁桃体细胞高十倍。
