Rabellino E M, Grey H M, LaForge S, Pirofsky B, Kashiwagi N, Malley A
J Clin Invest. 1976 Feb;57(2):390-7. doi: 10.1172/JCI108290.
Rhesus monkeys were immunized with normal human lymphoid cells, cultured lymphoid cells, and chronic leukemic lymphocytes. Antisera were analyzed by cytotoxicity and immunofluorescence techniques to study the antigenic characteristics of human lymphocytes. In an attempt to obtain a reagent specifically reactive with T (thymus-derived) lymphocytes, an antispleen antiserum was absorbed with cellf from five B- (bone marrow-derived) cell lines. After absorption, the antiserum killed 60-75% of peripheral blood lymphocytes and 40-50% of tonsil cells, so that there was a relationship between the percentage of killed cells and the proportion of T lymphocytes. However, when cells after cytotoxic treatment were assayed for rosette formation with sheep erythrocytes (a T-cell marker) 5-20% of viable rosette-forming lymphocytes were found. Therefore, this antiserum was cytotoxic for only 75-90% of T cells. From studies performed with antisera prepared against spleen and B-cell lines, we conclude that lymphoblastoid cells are antigenically different and deficient in comparison to normal B lymphocytes. In addition, cultured B-cell lines appear to be antigenically heterogenous, as shown by the cytotoxic activity remaining in antispleen and anti-B-cell lines sera after absorption with various numbers and types of lymphoid cell lines. After absorption with normal lymphocytes, an antiserum produced against chronic lymphatic leukemia cells had specific activity associated with 12 chronic lymphatic leukemia cells tested. Absorption of the same antiserum with leukemic cells from two patients showed that a certain degree of antigenic heterogeneity also exists among chronic leukemic lymphocytes.
用正常人淋巴细胞、培养的淋巴细胞和慢性白血病淋巴细胞对恒河猴进行免疫。通过细胞毒性和免疫荧光技术分析抗血清，以研究人类淋巴细胞的抗原特性。为了获得与T（胸腺衍生）淋巴细胞特异性反应的试剂，用来自五个B（骨髓衍生）细胞系的细胞吸收抗脾抗血清。吸收后，抗血清杀死了60 - 75%的外周血淋巴细胞和40 - 50%的扁桃体细胞，因此杀死细胞的百分比与T淋巴细胞的比例之间存在关系。然而，当用细胞毒性处理后的细胞检测与绵羊红细胞（一种T细胞标志物）的玫瑰花结形成时，发现有5 - 20%的存活玫瑰花结形成淋巴细胞。因此，这种抗血清仅对75 - 90%的T细胞具有细胞毒性。通过对针对脾脏和B细胞系制备的抗血清进行的研究，我们得出结论，淋巴母细胞在抗原性上与正常B淋巴细胞不同且存在缺陷。此外，培养的B细胞系似乎在抗原性上是异质的，如用各种数量和类型的淋巴细胞系吸收后，抗脾和抗B细胞系血清中仍保留的细胞毒性活性所示。用正常淋巴细胞吸收后，针对慢性淋巴细胞白血病细胞产生的抗血清对所测试的12个慢性淋巴细胞白血病细胞具有特异性活性。用两名患者的白血病细胞吸收同一抗血清表明，慢性白血病淋巴细胞之间也存在一定程度的抗原异质性。
