State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong, 271018, China; Biology Department, Dezhou College, Dezhou, 253023, China.
State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong, 271018, China.
Biochem Biophys Res Commun. 2019 Jun 4;513(3):582-588. doi: 10.1016/j.bbrc.2019.04.015. Epub 2019 Apr 10.
Heat stress significantly disturbs the protein folding and processing capability in plants. Molecular chaperones are vital players in unfolded/misfolded protein assembly and abiotic stress tolerance. Here, we reported SES1, which encodes an endoplasmic reticulum (ER) localized molecular chaperone, is required for Arabidopsis heat tolerance. SES1 is obviously induced by heat treatment and ses1 mutants are hypersensitive to heat stress. The unfolded protein response genes were up-regulated, while cytosolic protein response genes were down-regulated in ses1 after heat stress. Furthermore, ER stress sensor basic leucine zipper 28 (bZIP28) acts as the upstream transcriptional activator of SES1 by binding to its promoter region. These results provide new insights into heat stress responses and ER stress, and shed lights on the mechanism of SES1 in modulating heat resistance.
热应激会显著扰乱植物的蛋白质折叠和加工能力。分子伴侣是未折叠/错误折叠蛋白组装和非生物胁迫耐受的重要参与者。在这里，我们报道了 SES1，它编码一种内质网 (ER) 定位的分子伴侣，对于拟南芥的耐热性是必需的。SES1 明显受到热处理的诱导，ses1 突变体对热胁迫敏感。在热胁迫后，ses1 中的未折叠蛋白反应基因上调，而细胞质蛋白反应基因下调。此外，内质网应激传感器碱性亮氨酸拉链 28（bZIP28）通过结合其启动子区域作为 SES1 的上游转录激活因子。这些结果为热应激反应和内质网应激提供了新的见解，并阐明了 SES1 调节耐热性的机制。
