Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, Yunnan, China.
Department of Gynaecologic Oncology, The 3rd Affiliated Hospital of Kunming Medical University, Kunming 650118, China.
Infect Genet Evol. 2019 Sep;73:13-20. doi: 10.1016/j.meegid.2019.04.008. Epub 2019 Apr 11.
Human papillomavirus type 16 (HPV16) is a high-risk HPV subtype and a potent carcinogen. The HPV16 E6 and E7 genes are considered oncogenes that play a core role in the development of cervical cancer.
In the current study, we enrolled 97 HPV16-positive cervical cancer patients (case group) and 136 HPV16-positive asymptomatic individuals (control group) in a study to analyse the association between HPV16 E6 and E7 gene variations and cervical cancer.
Our results showed that three HPV16 sub-lineages (A1-A3, A4 and D3) were present; the distribution of these variants between the case and control group was not significantly different (P = 0.178). When the distribution of the HPV16 E6 and E7 gene variations was compared, the distribution of only A131C (R10R) in the E6 gene showed a different trend between the case and control groups and C749T (S63F) in the E7 gene was significantly different between the case and control groups (P = 0.071 and P = 4.861 × 10, respectively). Regarding the sub-lineages, no variations in the E6 gene were significantly different between the case and control group for the A4 (As) and A1-A3 (EUR) sub-lineages. However, the distribution of C749T (S63F) in the E7 gene was significantly different between the case and control groups for the A4 (As) and A1-A3 (EUR) sub-lineages (P = 1.815 × 10 and P = 0.008). In the current study, we found that the C749T (S63F) variation in the HPV16 E7 gene was associated with cervical cancer not only in the A4 (As) sub-lineage but also in the A1-A3 (EUR) sub-lineage.
Our study will provide a good reference for further functional studies of the relationship between cervical cancer carcinogenesis and the HPV16 E6 and E7 genes.
人乳头瘤病毒 16 型（HPV16）是一种高危 HPV 亚型，也是一种强有力的致癌物质。HPV16 的 E6 和 E7 基因被认为是致癌基因，在宫颈癌的发展中起着核心作用。
本研究纳入了 97 例 HPV16 阳性宫颈癌患者（病例组）和 136 例 HPV16 阳性无症状个体（对照组），旨在分析 HPV16 E6 和 E7 基因变异与宫颈癌之间的关系。
我们的研究结果显示，存在三种 HPV16 亚谱系（A1-A3、A4 和 D3）；病例组和对照组之间这些变体的分布没有显著差异（P=0.178）。当比较 HPV16 E6 和 E7 基因变异的分布时，E6 基因中只有 A131C（R10R）的分布在病例组和对照组之间表现出不同的趋势，而 E7 基因中的 C749T（S63F）在病例组和对照组之间存在显著差异（P=0.071 和 P=4.861×10-3）。关于亚谱系，在病例组和对照组之间，A4（As）和 A1-A3（EUR）亚谱系的 E6 基因变异没有显著差异。然而，E7 基因中的 C749T（S63F）分布在病例组和对照组之间 A4（As）和 A1-A3（EUR）亚谱系之间存在显著差异（P=1.815×10-3 和 P=0.008）。在本研究中，我们发现 HPV16 E7 基因中的 C749T（S63F）变异不仅与 A4（As）亚谱系，而且与 A1-A3（EUR）亚谱系的宫颈癌有关。
本研究为进一步研究 HPV16 E6 和 E7 基因与宫颈癌发生之间的关系提供了良好的参考。
