Iwanaga Toshihiko, Nio-Kobayashi Junko, Takahashi-Iwanaga Hiromi
Laboratory of Histology and Cytology, Department of Anatomy, Hokkaido University Graduate School of Medicine.
Biomed Res. 2019;40(2):79-85. doi: 10.2220/biomedres.40.79.
The vitreous of perinatal mice temporarily develops a unique vascular system, called the vasa hyaloidea propria (VHP). Observations showed the vessels possessed an extracellular matrix including the basement membrane in their entire length. Immunostaining of whole mount preparations of VHP with integrin β antibody displayed a bush-like network consisting of long and straight fibers which were associated with the VHP but extended apart from the blood vessels. Electron microscopically, each fiber was composed of a bundle of thin filaments different from collagen fibrils. Macrophages associated with the VHP appeared to be arrested by the integrin bushes. The integrin bushes fragmented and disappeared by postnatal day 10, just before the regression of the VHP. Macrophages were involved in the digestion and clearance of integrin bushes. The vitreous integrin bushes appear to provide a scaffold for architectural maintenance of the hyaloid vessels and macrophages.
围产期小鼠的玻璃体暂时发育出一种独特的血管系统，称为固有玻璃体血管（VHP）。观察表明，这些血管在其全长范围内都拥有包括基底膜在内的细胞外基质。用整合素β抗体对VHP整装标本进行免疫染色，显示出一个由长而直的纤维组成的灌木状网络，这些纤维与VHP相关，但从血管处延伸开来。电子显微镜下，每根纤维由一束不同于胶原纤维的细丝组成。与VHP相关的巨噬细胞似乎被整合素灌木所阻滞。在出生后第10天，就在VHP退化之前，整合素灌木破碎并消失。巨噬细胞参与了整合素灌木的消化和清除。玻璃体整合素灌木似乎为透明血管和巨噬细胞的结构维持提供了一个支架。
