Lou Y X
Biochimie. 1986 Oct-Nov;68(10-11):1237-43. doi: 10.1016/s0300-9084(86)80070-0.
A method of measuring kinetic parameters of immobilized enzyme with a recycling enzyme reactor system is described. By analyzing the plot of the dimensionless variable Ln(1-X)/X versus the time needed for a unit conversion, t/X, the mechanism of enzymatic reaction can be recognized and then its basic parameters can be evaluated. On the basis of the experimental data measured by P.R. Coulet et al., it has been proposed that the successive degradation of maltodextrins by the collagen membrane-bound amyloglucosidase was a product glucose inhibition reaction and their corresponding constants have been found with this method.
描述了一种利用循环酶反应器系统测量固定化酶动力学参数的方法。通过分析无量纲变量Ln(1-X)/X与单位转化所需时间t/X的关系图，可以识别酶促反应的机制，进而评估其基本参数。基于P.R. Coulet等人测得的实验数据，有人提出胶原膜结合的淀粉葡萄糖苷酶对麦芽糊精的连续降解是产物葡萄糖抑制反应，并已用该方法求出了相应的常数。
