Biopharmaceutical Development, MedImmune, Cambridge, UK.
GE Healthcare Bio-Sciences AB, BioProcess R&D, Uppsala, Sweden.
Biotechnol Prog. 2019 Jul;35(4):e2821. doi: 10.1002/btpr.2821. Epub 2019 May 8.
Perfusion is a cell culture mode that is gaining popularity for the manufacture of monoclonal antibodies and their derivatives. The cell culture media supporting perfusion culture need to support higher cell densities than those used in fed-batch culture. Therefore, when switching from a fed-batch to a perfusion mode, a new medium need to be developed which supports high cell densities, high productivity, and favorable product quality. We have developed a method for deriving perfusion culture media based on existing fed-batch media and feeds. We show that we can obtain culture media that successfully support perfusion cultures in a single-use rocking bioreactor system at cell-specific perfusion rates below 25 pL cell day . High productivities and favorable product quality are also achievable.
灌流是一种细胞培养方式，在用于单克隆抗体及其衍生物的生产方面越来越受欢迎。支持灌流培养的细胞培养基需要支持比补料分批培养更高的细胞密度。因此，当从补料分批培养切换到灌流模式时，需要开发一种新的培养基，该培养基支持高细胞密度、高生产力和良好的产品质量。我们已经开发了一种基于现有补料分批培养基和补料的灌流培养物培养基的衍生方法。我们表明，我们可以在一次性摇瓶生物反应器系统中以低于 25 pL 细胞天的细胞特异性灌流率成功获得支持灌流培养的培养基。还可以实现高生产力和良好的产品质量。
