a Faculty of Pharmacy, Federal University of Minas Gerais , Av. Pres. Antonio Carlos, 6627, Pampulla, Belo Horizonte, Minas Gerais , 31270-901 Brazil.
b Biomedical Laboratory Diagnostics, Michigan State University , 354 Farm Lane, East Lansing , Michigan 48824.
Mycologia. 2019 May-Jun;111(3):408-422. doi: 10.1080/00275514.2019.1589316. Epub 2019 Apr 15.
We studied phylogenetic and taxonomic features of isolates identified as recovered from six different species of mosquito larvae. The isolates grew vigorously at 25 C, moderately at 30 C, and not at all at 37 C and developed submerged, white colonies with few short, hyaline, aerial hyphae. Cultures displayed phenotypic plasticity, with broad, hyaline hyphae strongly constricted at septa that developed oval, spherical, or amorphous segments. These developed into sporangia producing one or two exit tubes, from which evanescent gelatinous vesicles containing zoospores developed. Three isolates developed oogonia consistent with features previously described for . Phylogenetic analysis of nuc rDNA internal transcribed spacer (ITS1-5.8S-ITS = ITS) and cytochrome oxidase subunit II () sequences of consistently grouped into eight clusters. Four of the investigated isolates grouped with sequences of an unnamed species infecting nematodes. Based on phenotypic and phylogenetic data, we describe the latter isolates as , sp. nov. In addition, we also investigated a species of from a dog with lagenidiosis and describe it as new, , sp. nov.
我们研究了从六种不同蚊幼虫中分离出的菌株的系统发育和分类特征。这些分离株在 25°C 下生长旺盛，在 30°C 下生长适度，在 37°C 下则完全不生长，形成了白色的浸没状菌落，具有少量短而透明的气生菌丝。培养物表现出表型可塑性，具有宽而透明的菌丝，在隔膜处强烈收缩，形成椭圆形、球形或无定形的节段。这些节段发育成产生一个或两个出口管的孢子囊，从中发育出含有游动孢子的瞬态凝胶状囊泡。有 3 个分离株发育成与先前描述的特征一致的卵菌。ITS1-5.8S-ITS（ITS）区和细胞色素氧化酶亚基 II（）区核 rDNA 的系统发育分析结果一致，将 8 个簇分组。其中 4 个被调查的分离株与感染线虫的未命名物种的序列聚为一组。基于表型和系统发育数据，我们将后一种分离株描述为新种，即。此外，我们还研究了一种来自患有lagenidiosis 的狗的物种，并将其描述为新种，即。
