一种与黑腹果蝇18 - 28 S核糖体DNA非转录间隔区相关的独特核蛋白结构。

A unique nucleoprotein structure associated with the Drosophila melanogaster 18-28 S rDNA nontranscribed spacer.

作者信息

Levinger L F, Nass G S

出版信息

FEBS Lett. 1986 Dec 15;209(2):340-6. doi: 10.1016/0014-5793(86)81139-5.

Abstract

We have detected unique nucleoprotein particles specific for the 18-28 S rDNA nontranscribed spacer of Drosophila melanogaster. The particles migrate between di- and trinucleosomes on nucleoprotein gels, and are between mono- and dinucleosomal in DNA length. These migration properties suggest that the nontranscribed spacer particles could have a protein component larger than a histone core. The variant nucleoprotein structures map primarily within the nontranscribed spacer 235 base pair internal subrepeat, which is AT-rich and possesses a 50 base pair sequence homologous to the RNA polymerase I binding site.

摘要

我们已经检测到了特异于黑腹果蝇18 - 28 S rDNA非转录间隔区的独特核蛋白颗粒。这些颗粒在核蛋白凝胶上的二核小体和三核小体之间迁移，其DNA长度介于单核小体和双核小体之间。这些迁移特性表明，非转录间隔区颗粒可能具有比组蛋白核心更大的蛋白质成分。这些变异的核蛋白结构主要定位在非转录间隔区235个碱基对的内部亚重复序列内，该序列富含AT，并拥有一段与RNA聚合酶I结合位点同源的50个碱基对的序列。