Department of Chemical Engineering, University of Chemistry and Technology, Prague, Technická 5, 166 28 Praha 6, Czech Republic.
Phys Chem Chem Phys. 2019 Apr 24;21(17):8619-8622. doi: 10.1039/c9cp00630c.
Herein, we present direct experimental evidence of pH oscillatory dynamics in the urea-urease enzymatic reaction conducted in a continuous reactor-membrane-reservoir system. Our results are consistent with earlier model predictions requiring differential transport of H+ and substrate. We report oscillations with periods in hundreds of seconds and the amplitude of ∼0.1 pH units.
在此，我们在连续式反应器-膜-储液系统中进行尿素-脲酶酶反应，提供了 pH 振荡动力学的直接实验证据。我们的结果与先前需要 H+和底物的差分传输的模型预测一致。我们报告的振荡周期为数秒至数百秒，幅度约为 0.1 pH 单位。
