Hernández-Muñiz W, Stevens S E
J Bacteriol. 1987 Jan;169(1):218-23. doi: 10.1128/jb.169.1.218-223.1987.
The cyanobacterium Mastigocladus laminosus produces motile hormogonia which move by gliding motility. These hormogonia were characterized in terms of their morphology, state of differentiation of the cells, optimal temperature for production and motility, minimal nutritional requirements to sustain motility, liberation of the hormogonium from its parental trichome, average surface velocity, and maximal concentration of agar through which the hormogonium may move. We found that an average hormogonium consisted of 13.6 cells of only the narrow-cell-type morphology. Gliding motility and the production of hormogonia were maximal at 45 degrees C. Agarose plus 0.20 mM Ca2+ was sufficient to sustain gliding motility. Hormogonia were liberated from the parental trichome by formation and lysis of a necridium. The average surface velocity of a hormogonium was 1.7 micron/s with a maximal velocity of 3 micron/s. Hormogonia were motile through 7% agar. Motile hormogonia leave a record of their passage in the form of easily visible tracks on the surface of solid media. Three types of tracks were observed: straight, sinusoidal, and circular. Normal, forward-directed motion involves screwlike rotation that describes a right-handed helix. However, observations are presented which suggest that rotational motion is not a prerequisite for gliding motility in this cyanobacterium.
蓝藻层理鞭枝藻产生可运动的藻殖段，它们通过滑行运动移动。对这些藻殖段在形态、细胞分化状态、产生和运动的最佳温度、维持运动所需的最低营养需求、藻殖段从亲代丝状体中释放、平均表面速度以及藻殖段可移动通过的琼脂最大浓度等方面进行了表征。我们发现，一个平均的藻殖段由仅呈窄细胞型形态的13.6个细胞组成。滑行运动和藻殖段的产生在45摄氏度时达到最大值。琼脂糖加0.20 mM Ca2+足以维持滑行运动。藻殖段通过坏死体的形成和裂解从亲代丝状体中释放出来。藻殖段的平均表面速度为1.7微米/秒，最大速度为3微米/秒。藻殖段能在7%的琼脂中运动。可运动的藻殖段在固体培养基表面留下易于观察到的轨迹记录其移动路径。观察到三种类型的轨迹：直线型、正弦型和圆形。正常的向前运动涉及呈右手螺旋的螺旋状旋转。然而，所呈现的观察结果表明，旋转运动并非该蓝藻滑行运动的先决条件。
