Key Laboratory of Chemical Safety and Health, Chinese Center for Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 100050 Beijing, China.
National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, 100050 Beijing, China.
J Food Drug Anal. 2019 Apr;27(2):518-525. doi: 10.1016/j.jfda.2019.03.001. Epub 2019 Mar 23.
Benzo [a]pyrene (BaP) is a model compound for the study of polycyclic aromatic hydrocarbon (PAH) carcinogenesis. Upon metabolism, BaP is metabolized to the ultimate metabolite, BaP trans-7,8-diol-anti-9,10-epoxide (BPDE), that reacts with cellular DNA to form BPDE-dG adducts responsible for BaP-induced mutagenicity, carcinogenicity, and teratogenicity. In this study, we employed our developed LC-MS/MS method to detect and quantity BPDE-dG adducts present in 42 normal human umbilical cord blood samples and 42 birth defect cases. We determined that there is no significant difference in the level of BPDE-dG formation between the normal and birth defect groups. This represents the first time to use an LC-MS/MS method to quantify BPDE-dG in human umbilical blood samples. The results indicated that under experimental conditions, BPDE-dG adducts were detected in all the human umbilical cord blood samples from the normal and birth defect groups.
苯并[a]芘（BaP）是多环芳烃（PAH）致癌作用研究的模型化合物。BaP 经代谢后生成最终代谢物 BaP 反-7,8-二醇-顺-9,10-环氧化物（BPDE），BPDE 与细胞 DNA 反应形成 BPDE-dG 加合物，导致 BaP 诱导的突变性、致癌性和致畸性。在本研究中，我们采用开发的 LC-MS/MS 方法检测并定量 42 例正常脐带血样本和 42 例出生缺陷病例中存在的 BPDE-dG 加合物。结果表明，正常组和出生缺陷组 BPDE-dG 的形成水平无显著差异。这是首次采用 LC-MS/MS 方法定量检测人脐血样本中的 BPDE-dG。结果表明，在实验条件下，正常组和出生缺陷组的所有脐带血样本中均检测到 BPDE-dG 加合物。
