Dickson G R
Histochemistry. 1978 Sep 28;57(4):343-7. doi: 10.1007/BF00492669.
The ultrastructural localization of alkaline phosphatase was studied in the hypertrophic chondrocyte of the frog (Rana temporaria) by incubating sections of glutaraldehyde fixed tissue in a medium containing sodium beta glycerophosphate and calcium chloride. Control specimens were incubated in substrate free medium. Alkaline phosphatase (orthophosphoric monoester phosphohydrolase) is a high molecular weight glycoprotein that hydrolyses phosphorylated metabolites much as acid phosphatase does except that its action is optimal at an alkaline pH. The results of this investigation showed that alkaline phosphatase activity was present within the cytoplasm and around the plasma membrane of frog hypertrophic chondrocytes. Although only a small proportion of frog hypertrophic chondrocytes demonstrated enzyme activity, there was evidence that this was concentrated within Golgi lamellae and vesicles leaving other organelles unreactive. The finding of alkaline phosphatase activity within Golgi lamellae of hypertrophic chondrocytes is regarded as unusual although postitive reactions within chondrocyte lysosomes have previously been reported (Doty and Schofield, 1976).
通过将戊二醛固定组织的切片置于含有β-甘油磷酸钠和氯化钙的培养基中孵育，研究了青蛙（林蛙）肥大软骨细胞中碱性磷酸酶的超微结构定位。对照标本在无底物培养基中孵育。碱性磷酸酶（正磷酸单酯磷酸水解酶）是一种高分子量糖蛋白，它水解磷酸化代谢物的方式与酸性磷酸酶相似，只是其作用在碱性pH值下最适宜。本研究结果表明，青蛙肥大软骨细胞的细胞质内和质膜周围存在碱性磷酸酶活性。虽然只有一小部分青蛙肥大软骨细胞显示出酶活性，但有证据表明这种活性集中在高尔基体片层和小泡内，而其他细胞器没有反应。肥大软骨细胞高尔基体片层内发现碱性磷酸酶活性被认为是不寻常的，尽管此前曾报道过软骨细胞溶酶体内有阳性反应（多蒂和斯科菲尔德，1976年）。
