Wei Wenhao, Lv Yufeng, Gan Zuhuan, Zhang Yanxian, Han Xueqiong, Xu Zihai
Department of Medical Oncology, Affiliated Langdong Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China.
Department of Medical Oncology, The First People's Hospital of Nanning, Nanning, Guangxi Zhuang Autonomous Region 530022, P.R. China.
Oncol Lett. 2019 May;17(5):4321-4328. doi: 10.3892/ol.2019.10130. Epub 2019 Mar 8.
Clear cell renal cell carcinoma (ccRCC) is the most common and lethal renal malignant tumor in adults. The aim of the present study was to identify the key genes involved in ccRCC metastasis. Expression profiling data for ccRCC patients with metastasis and without metastasis were obtained from The Cancer Genome Atlas database. The datasets were used to identify differentially expressed genes (DEGs) between the metastasis group and the non-metastasis group using the DESeq2 package. Function enrichment analyses of DEGs were performed. The protein-protein interaction (PPI) network was constructed and analyzed using the Search Tool for the Retrieval of Interacting Genes and Cytoscape for further analysis of the identified hub genes. A total of 472 DEGs were identified, including 247 that were upregulated and 225 that were downregulated in the metastasis group. Gene Ontology enrichment analysis revealed that DEGs were mainly enriched in cell transmembrane movement and mitotic cell cycle process. Kyoto Encyclopedia of Genes Genomes pathway analysis revealed that the DEGs were mainly involved in the 'cell cycle' (hsa04110), 'collecting duct acid secretion' (hsa04966), 'complement and coagulation cascades' (hsa04610) and 'aldosterone-regulated sodium reabsorption' (hsa04960) pathways. Using the PPI network, 35 hub genes were identified, and the majority of them were upregulated in ccRCC tissue compared with normal kidney tissue. The expression levels of certain hub genes (CDKN3, TPX2, BUB1B, CDCA8, UBE2C, NDC80, RRM2, NCAPG, NCAPH, PTTG1, FAM64A, ANLN, KIF4A, CEP55, CENPF, KIF20A, ASPM and HJURP) were significantly associated with overall survival and recurrence-free survival in ccRCC. The present study has identified key genes associated with the metastasis of ccRCC.
透明细胞肾细胞癌（ccRCC）是成人中最常见且致命的肾脏恶性肿瘤。本研究的目的是鉴定参与ccRCC转移的关键基因。从癌症基因组图谱数据库获取了有转移和无转移的ccRCC患者的表达谱数据。使用DESeq2软件包来鉴定转移组和非转移组之间的差异表达基因（DEG）。对DEG进行了功能富集分析。使用检索相互作用基因的搜索工具和Cytoscape构建并分析了蛋白质-蛋白质相互作用（PPI）网络，以进一步分析鉴定出的枢纽基因。共鉴定出472个DEG，其中转移组中有247个上调，225个下调。基因本体富集分析显示，DEG主要富集于细胞跨膜运动和有丝分裂细胞周期过程。京都基因与基因组百科全书通路分析显示，DEG主要参与“细胞周期”（hsa04110）、“集合管酸分泌”（hsa04966）、“补体和凝血级联反应”（hsa04610）以及“醛固酮调节的钠重吸收”（hsa04960）通路。利用PPI网络，鉴定出35个枢纽基因，与正常肾组织相比，它们中的大多数在ccRCC组织中上调。某些枢纽基因（CDKN3、TPX2、BUB1B、CDCA8、UBE2C、NDC80、RRM2、NCAPG、NCAPH、PTTG1、FAM64A、ANLN、KIF4A、CEP55、CENPF、KIF20A、ASPM和HJURP）的表达水平与ccRCC的总生存期和无复发生存期显著相关。本研究鉴定出了与ccRCC转移相关的关键基因。
