Tykarska E, Lebioda L, Bradshaw T P, Dunlap R B
J Mol Biol. 1986 Sep 5;191(1):147-50. doi: 10.1016/0022-2836(86)90433-x.
Several new crystal forms of thymidylate synthase (5,10-methlenetetrahydrofolate:dUMP C-methyltransferase; EC 2.1.1.45) were obtained by controlled pH change. In the crystals the dimeric molecule has a 2-fold symmetry axis coinciding with crystallographic symmetry. The crystals scatter to at least 2.7 A resolution in the synchrotron X-ray beam and appear to be suitable for high-resolution X-ray diffraction analysis. The crystals were successfully derivatized and preliminary results are reported for the covalent inhibitory ternary complex of thymidylate synthase, 5-fluoro-2'-deoxyuridylate and 5,10-methylenetetrahydrofolate.
通过控制pH值变化获得了胸苷酸合成酶（5,10-亚甲基四氢叶酸：dUMP C-甲基转移酶；EC 2.1.1.45）的几种新晶型。在晶体中，二聚体分子具有与晶体学对称性重合的二次对称轴。这些晶体在同步加速器X射线束中散射至至少2.7 Å分辨率，似乎适合进行高分辨率X射线衍射分析。晶体已成功衍生化，并报告了胸苷酸合成酶、5-氟-2'-脱氧尿苷酸和5,10-亚甲基四氢叶酸的共价抑制三元复合物的初步结果。
