Liu Hong-Jie, Dong Han-Qiu, Zhan Sha, Chen Liang, Xiao Ya
College of Traditional Chinese Medicine,Ji'nan University Guangzhou 510632,China.
Zhongguo Zhong Yao Za Zhi. 2019 Feb;44(4):827-832. doi: 10.19540/j.cnki.cjcmm.20181128.006.
This study based on1H-NMR urine metabolomics technique combined with biochemical indicators to focus on studying the acute hepatotoxicity mechanism of Artemisia argyi essential oil( AAEO). In order to further explore the acute hepatotoxicity mechanism of AAEO,the researchers collected the urine nuclear magnetic data of rats in different periods of high and low doses of olive oil and AAEO group. Using the principal component analysis( PCA) and orthogonal partial least squares-discrimination analysis( OPLSDA) to analyze the endogenous small molecule metabolites in rat urine to study the effects of AAEO on the metabolic process of normal rats. The results showed there was a significant difference between the olive oil group and the AAEO group,the PCA scores chart demonstrated that there was no obvious separation tendency in the urine of olive oil group rats 0-6,6-12,12-24 h,and the metabolic components were distributed in aggregation pattern. The urinary metabolic trajectory of the rats in the AAEO group was conspicuously separated at 0-6,6-12,12-24 h. The experiments proved that the analysis of metabolites by1H-NMR found that AAEO caused metabolic disorders in rats and produced acute hepatotoxicity. After metabolite differential comparison,it was speculated that the mechanism of acute hepatotoxicity may be involved in the tricarboxylic acid cycle and energy metabolism,while the citrate and oleanolic acid would be the potential biomarkers. This study discussed that the acute hepatotoxicity mechanism of AAEO was used to provide the experimental data for the clinical prescription of Artemisia argyi.
本研究基于1H-NMR尿液代谢组学技术结合生化指标，重点研究艾叶精油（AAEO）的急性肝毒性机制。为进一步探究AAEO的急性肝毒性机制，研究人员收集了高、低剂量橄榄油组和AAEO组大鼠不同时间段的尿液核磁数据。采用主成分分析（PCA）和正交偏最小二乘判别分析（OPLSDA）对大鼠尿液中的内源性小分子代谢物进行分析，以研究AAEO对正常大鼠代谢过程的影响。结果显示，橄榄油组与AAEO组之间存在显著差异，PCA得分图表明，橄榄油组大鼠在0 - 6、6 - 12、12 - 24 h尿液中无明显分离趋势，代谢成分呈聚集分布。AAEO组大鼠在0 - 6、6 - 12、12 - 24 h的尿液代谢轨迹明显分离。实验证明，通过1H-NMR对代谢物进行分析发现，AAEO可导致大鼠代谢紊乱并产生急性肝毒性。经代谢物差异比较推测，急性肝毒性机制可能涉及三羧酸循环和能量代谢，而柠檬酸和齐墩果酸可能是潜在生物标志物。本研究探讨了AAEO的急性肝毒性机制，为艾叶临床用药提供实验数据。
