Key Laboratory of Advanced Drug Preparation Technologies, Ministry of Education of China, School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, China.
Henan Joint International Research Laboratory of Chronic Liver Injury, Henan Key Laboratory of Rehabilitation Medicine, Department of Pediatrics, the Fifth Affiliated Hospital, Zhengzhou University, Zhengzhou, China.
Biomed Chromatogr. 2024 Apr;38(4):e5826. doi: 10.1002/bmc.5826. Epub 2024 Jan 11.
Artemisia argyi H.Lév. & Vaniot essential oil (AAEO) has shown pharmacological effects such as anti-inflammation, antioxidant, and anti-tumor properties. However, the protective effect of AAEO on lipopolysaccharide (LPS)-induced liver injury and its potential protective mechanism are still unclear. In this study, we used ultra-performance liquid chromatography tandem mass spectrometry metabolomics techniques to investigate the changes in liver tissue metabolites in mice exposed to LPS with or without AAEO treatment for 14 days. The biochemical results showed that compared with the control group, AAEO significantly reduced the levels of liver functional enzymes, suggesting a significant improvement in liver injury. In addition, the 18 differential metabolites identified by metabolomics were mainly involved in the reprogramming of arachidonic acid metabolism, tryptophan metabolism, and purine metabolism. AAEO could significantly inhibit the expression of COX-2, IDO1, and NF-κB; enhance the body's anti-inflammatory ability; and alleviate liver injury. In summary, our study identified the protective mechanism of AAEO on LPS-induced liver injury at the level of small molecular metabolites, providing a potential liver protective agent for the treatment of LPS-induced liver injury.
艾蒿精油（AAEO）具有抗炎、抗氧化、抗肿瘤等药理作用。然而，AAEO 对脂多糖（LPS）诱导的肝损伤的保护作用及其潜在的保护机制尚不清楚。在本研究中，我们使用超高效液相色谱串联质谱代谢组学技术，研究了 LPS 暴露后 14 天给予或不给予 AAEO 治疗的小鼠肝组织代谢物的变化。生化结果表明，与对照组相比，AAEO 显著降低了肝功能酶的水平，提示肝损伤有明显改善。此外，通过代谢组学鉴定的 18 种差异代谢物主要涉及花生四烯酸代谢、色氨酸代谢和嘌呤代谢的重编程。AAEO 能显著抑制 COX-2、IDO1 和 NF-κB 的表达，增强机体抗炎能力，减轻肝损伤。综上所述，本研究在小分子代谢物水平上确定了 AAEO 对 LPS 诱导的肝损伤的保护机制，为治疗 LPS 诱导的肝损伤提供了一种潜在的肝保护剂。
