基于DNA条形码技术鉴定23种黎族未知药用植物

[Identification of 23 unknown Li minority medicinal plants based on DNA barcoding].

作者信息

Cui Xin-Yun, Sun Wei, Xiong Chao, Meng Xiang-Xiao, Shi Yu-Hua, Wu Lan, Cheng Li-Li, Li Wei-Jie, Zheng Xi-Long

机构信息

Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine,Hainan Branch of the Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College Haikou 570311,China.

Key Laboratory of Beijing for Identification and Safe Evaluation of Chinese Medicine,Institute of Chinese Meteria Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2019 Jan;44(2):283-292. doi: 10.19540/j.cnki.cjcmm.20181106.002.

DOI:10.19540/j.cnki.cjcmm.20181106.002

PMID:30989947

Abstract

DNA barcode molecular biological technique is used to identify the species of 23 unknown Li minority medicinal plants.DNA was extracted from 23 unknown medicines using the Plant Genomic DNA Extraction kit. The ITS2 and psbA-trnH regions were amplified and sequenced bi-directionally. The Codon Code Aligner V 7. 0. 1 was used to proofread and assemble the contigs and generated consensus sequences. All the sequences were submitted to Traditional Chinese Medicine DNA Barcode Database and NCBI Gen Bank to get information of the species identifications. If the maximum similarity of the identification result is ≥ 97%,exact species can be known. If it is between 97% and 90%,samples' genus can be confirmed; If it is <90%,then we can only confirm its family. Finally there are 17 samples can be identified to species level,5 can be identified to genus level and 1 can be identified to family level. This shows that DNA barcoding used in medicinal plants molecular identification,can identify unknown species rapidly and accurately.

摘要

采用DNA条形码分子生物学技术对23种未知的黎族药用植物进行物种鉴定。使用植物基因组DNA提取试剂盒从23种未知药物中提取DNA。对ITS2和psbA-trnH区域进行双向扩增和测序。使用Codon Code Aligner V 7.0.1对重叠群进行校对和组装，并生成一致序列。将所有序列提交至中药DNA条形码数据库和NCBI基因库以获取物种鉴定信息。若鉴定结果的最大相似度≥97%，则可确定具体物种；若在97%至90%之间，则可确认样本的属；若<90%，则只能确认其科。最终有17个样本可鉴定到物种水平，5个可鉴定到属水平，1个可鉴定到科水平。这表明DNA条形码技术用于药用植物分子鉴定，能够快速、准确地鉴定未知物种。