College of Chinese Medicine Materials, Jilin Agriculture University, Changchun 130118, China; Institute of Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, China.
Institute of Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, China.
Chin J Nat Med. 2019 Aug;17(8):585-590. doi: 10.1016/S1875-5364(19)30060-3.
The aim is to select a universal DNA barcode for identifying all poisonous medicinal plants in Chinese pharmacopoeia and their poisonous related species or adulterants. We chose 4 commonly used regions as candidate DNA barcodes (ITS2, psbA-trnH, matK and rbcL) and compared their identification efficiency in 106 species from 27 families and 65 genera totally. Data analysis was performed including the information of sequence alignment, inter/intra-specific genetic distance and data distribution, identification efficiency and the situation of Neighbor-Joining (NJ) phylogenetic trees. We found ITS2 sequence region had high variation, stable genetic distance and identification efficiency relatively. The topological structure of NJ phylogenetic tree showed monophyletic. Our findings show that ITS2 can be applied as a universal barcode for identifying poisonous medicinal plants in Chinese pharmacopoeia and their poisonous related species or adulterants.
目的是选择一个通用的 DNA 条码来鉴定中国药典中的所有有毒药用植物及其有毒相关物种或掺杂物。我们选择了 4 个常用区域作为候选 DNA 条码（ITS2、psbA-trnH、matK 和 rbcL），并比较了它们在 27 科 65 属 106 种中的鉴定效率。数据分析包括序列比对信息、种内/种间遗传距离和数据分布、鉴定效率以及邻接法（NJ）系统发育树的情况。我们发现 ITS2 序列区域具有较高的变异性、相对稳定的遗传距离和鉴定效率。NJ 系统发育树的拓扑结构显示为单系性。我们的研究结果表明，ITS2 可作为一种通用条码，用于鉴定中国药典中的有毒药用植物及其有毒相关物种或掺杂物。
