痉挛多肽表达性化生与 miR-21、155 和 223 的高表达相关，可通过幽门螺杆菌根除在胃癌家族亲属中逆转。

Spasmolytic polypeptide-expressing metaplasia associated with higher expressions of miR-21, 155, and 223 can be regressed by Helicobacter pylori eradication in the gastric cancer familial relatives.

机构信息

Institute of Clinical Medicine, College of Medicine, National Cheng Kung University Hospital, National Cheng Kung University, Tainan, Taiwan.

Department of Internal Medicine, College of Medicine, National Cheng Kung University Hospital, National Cheng Kung University, Tainan, Taiwan.

出版信息

Helicobacter. 2019 Jun;24(3):e12578. doi: 10.1111/hel.12578. Epub 2019 Apr 16.

DOI:10.1111/hel.12578

PMID:30990573

Abstract

BACKGROUND AND AIMS

Spasmolytic polypeptide-expressing metaplasia (SPEM) is a preneoplastic gastric cancer lesion related to epigenetic microRNA (miRNA) expression. This study elucidated whether Helicobacter pylori-infected first-degree relatives of patients with gastric cancer (GCF) are susceptible to have SPEM and correlated with miR-21, 155, and 223 expressions. We also validated whether SPEM and these miRNAs can be regressed after H pylori eradication.

METHODS

We prospectively enrolled 148 GCF and 148 nonulcer dyspepsia (NUD) subjects without gastric cancer familial history as controls. Each case had received a panendoscopy to determine H pylori status and gastric histology, including SPEM. The cases with SPEM were followed after H pylori eradication to determine SPEM regression. The total RNA was extracted to analyze tissues miR-21, 155, and 223 before and after eradication.

RESULTS

GCF subjects had a higher prevalence of H pylori infection (73% vs 32%) and SPEM (42% vs 14%, P < 0.01) than controls. The tissue miR-21, 155, and 223 in antrum were higher in cases with SPEM than in those without SPEM (P <= 0.05). There was similar SPEM reversibility after H pylori eradication between GCF subjects and controls (72% vs 69%, P = 0.852). In the SPEM regressed cases, tissue miR-21, 155, and 223 decreased after H pylori eradication (P < 0.05).

CONCLUSION

The H pylori-infected GCF subjects were prone to have SPEM with higher tissues miR-21, 155, and 223 expressions. H pylori eradication can result in a 70% SPEM regression, accompanied by a decline in miR-21, 155, and 233 expression levels.

摘要

背景和目的

痉挛多肽表达化生（SPEM）是一种与表观遗传 microRNA（miRNA）表达相关的胃癌前病变。本研究阐明了胃癌患者的 H. pylori 感染一级亲属是否易发生 SPEM，并与 miR-21、155 和 223 的表达相关。我们还验证了 SPEM 和这些 miRNA 是否可以在 H. pylori 根除后消退。

方法

我们前瞻性纳入了 148 例胃癌患者的一级亲属（GCF）和 148 例非溃疡性消化不良（NUD）患者作为对照，这些患者均无胃癌家族史。每位患者均接受了全内镜检查以确定 H. pylori 状态和胃组织学，包括 SPEM。对有 SPEM 的病例进行 H. pylori 根除后随访，以确定 SPEM 消退情况。在根除前后提取总 RNA，分析组织 miR-21、155 和 223。

结果

GCF 组 H. pylori 感染率（73% vs. 32%）和 SPEM 发生率（42% vs. 14%，P<0.01）均高于对照组。有 SPEM 的病例胃窦组织 miR-21、155 和 223 高于无 SPEM 的病例（P<0.05）。GCF 组和对照组的 SPEM 根除后逆转率相似（72% vs. 69%，P=0.852）。在 SPEM 消退的病例中，H. pylori 根除后组织 miR-21、155 和 223 降低（P<0.05）。