Pekary A E, Reeve J R, Smith V P
Life Sci. 1986 Dec 29;39(26):2565-70. doi: 10.1016/0024-3205(86)90110-4.
TRH occurs in very high concentration in rat prostate. A species specific protein with repetitive -Gln-His-Pro-Gly- sequences, which are flanked on the N- and C-terminus by paired basic residues, has been shown to be the source of TRH in frog skin and rat hypothalamus. Following cleavage by trypsin-like enzymes, the peptide fragments with N-terminal Gln spontaneously cyclize to pGlu while Gly within the C-terminally extended peptides serves as the -NH2 donor for the alpha-amidation of the proline residue. Because this last step in the biosynthesis of TRH is rate limiting for pGlu-His-Pro-Gly, we have combined several chromatographic and radioimmunoassay techniques to identify this TRH precursor in rat prostate.
促甲状腺激素释放激素（TRH）在大鼠前列腺中浓度极高。一种具有重复的-Gln-His-Pro-Gly-序列的物种特异性蛋白质，其在N端和C端两侧均为成对的碱性残基，已被证明是青蛙皮肤和大鼠下丘脑TRH的来源。经类胰蛋白酶酶切割后，N端为Gln的肽片段自发环化为焦谷氨酸（pGlu），而C端延伸肽内的Gly作为脯氨酸残基α-酰胺化的-NH2供体。由于TRH生物合成的最后一步是pGlu-His-Pro-Gly的限速步骤，我们结合了多种色谱和放射免疫分析技术来鉴定大鼠前列腺中的这种TRH前体。
