体内用抗原修饰的同基因细胞进行免疫抑制。I. 针对三元共聚物聚（谷氨酸、赖氨酸、苯丙氨酸）n的T细胞介导的抑制作用。
Immune suppression in vivo with antigen-modified syngeneic cells. I. T-cell-mediated suppression to the terpolymer poly-(Glu, Lys, Phe)n.
作者信息
Cheung N K, Scherr D H, Heghinian K M, Benacerraf B, Dorf M E
出版信息
J Exp Med. 1978 Dec 1;148(6):1539-49. doi: 10.1084/jem.148.6.1539.
Abstract
The palmitoyl derivative of the linear polypeptide of poly-(L-Glu-L-Lys-L-Phe)n (GLphi) can be coupled to spleen cells directly. The intravenous administration of 2 X 10(5)--3 X 10(7) GLphi-coupled syngeneic spleen cells induces GL-phi-specific suppressor T cells in C57BL/6 nonresponder mice. The suppression is antigen specific and can be detected by the inhibition of the primary GLphi plaque-forming cell response to challenge with GLphi-fowl gamma globulin. The number of inducer cells required for suppression carry less than 0.1 microgram of antigen. Spleen cells from tolerized mice can transfer suppression to normal syngeneic recipients. The suppression is cyclophosphamide sensitive and the suppressor cells bear the Thy 1.2 marker. This method of inducing antigen-specific suppressor cells may be generally applicable to other antigen systems.
摘要
聚（L-谷氨酸-L-赖氨酸-L-苯丙氨酸）n（GLphi）线性多肽的棕榈酰衍生物可直接与脾细胞偶联。静脉注射2×10⁵ - 3×10⁷个GLphi偶联的同基因脾细胞可在C57BL/6无反应小鼠中诱导产生GLphi特异性抑制性T细胞。这种抑制是抗原特异性的，可通过抑制初次GLphi空斑形成细胞对GLphi - 鸡γ球蛋白攻击的反应来检测。产生抑制所需的诱导细胞数量携带的抗原少于0.1微克。来自耐受小鼠的脾细胞可将抑制作用转移给正常同基因受体。这种抑制对环磷酰胺敏感，且抑制性细胞带有Thy 1.2标记。这种诱导抗原特异性抑制细胞的方法可能普遍适用于其他抗原系统。
相似文献
1
Immune suppression in vivo with antigen-modified syngeneic cells. I. T-cell-mediated suppression to the terpolymer poly-(Glu, Lys, Phe)n.体内用抗原修饰的同基因细胞进行免疫抑制。I. 针对三元共聚物聚（谷氨酸、赖氨酸、苯丙氨酸）n的T细胞介导的抑制作用。
J Exp Med. 1978 Dec 1;148(6):1539-49. doi: 10.1084/jem.148.6.1539.
2
3
Gene complementation in the T-lymphocyte proliferative response to poly (Glu55Lys36Phe9)n. A demonstration that both immune response gene products must be expressed in the same antigen-presenting cell.T淋巴细胞对聚（谷氨酸55-赖氨酸36-苯丙氨酸9）n增殖反应中的基因互补作用。证明两种免疫反应基因产物必须在同一个抗原呈递细胞中表达。
J Exp Med. 1979 Jan 1;149(1):40-57. doi: 10.1084/jem.149.1.40.
4
Regulation of antibody heterogeneity by suppressor T cells: diminishing suppressor T cell activity increases the number of dinitrophenyl clones in mice immunized with dinitrophenyl-poly(Glu,Lys,Phe) or dinitrophenyl-poly(Glu,Lys,Ala).抑制性T细胞对抗体异质性的调节：降低抑制性T细胞活性会增加用二硝基苯基-聚（谷氨酸、赖氨酸、苯丙氨酸）或二硝基苯基-聚（谷氨酸、赖氨酸、丙氨酸）免疫的小鼠中二硝基苯基克隆的数量。
Proc Natl Acad Sci U S A. 1978 Jun;75(6):2914-7. doi: 10.1073/pnas.75.6.2914.
5
In vivo effects of anti-Ia alloantisera. I. Elimination of specific suppression by in vivo administration of antisera specific for I-J controlled determinants.抗Ia同种异体抗血清的体内效应。I. 通过体内给予针对I-J控制决定簇的特异性抗血清消除特异性抑制。
J Exp Med. 1978 Mar 1;147(3):656-66. doi: 10.1084/jem.147.3.656.
6
Antigen-specific T cell clones restricted to unique F1 major histocompatibility complex determinants. Inhibition of proliferation with monoclonal anti-Ia antibody.受限于独特F1主要组织相容性复合体决定簇的抗原特异性T细胞克隆。用单克隆抗Ia抗体抑制增殖。
J Exp Med. 1981 Mar 1;153(3):677-93. doi: 10.1084/jem.153.3.677.
7
8
Genetic control of specific immune suppression. IV. Responsiveness to the random copolymer L-glutamic acid50-L-tyrosine50 induced in BALB/c mice by cyclophosphamide.特异性免疫抑制的遗传控制。IV. 环磷酰胺诱导BALB/c小鼠对随机共聚物L-谷氨酸50-L-酪氨酸50的反应性。
J Exp Med. 1976 Jul 1;144(1):277-81. doi: 10.1084/jem.144.1.277.
9
Gene complementation. Neither Ir-GLphi gene need be present in the proliferative T cell to generate an immune response to Poly(Glu55Lys36Phe9)n.基因互补。在增殖性T细胞中，无需存在任何一个Ir - GLphi基因即可对聚（Glu55Lys36Phe9）n产生免疫反应。
J Exp Med. 1980 Jun 1;151(6):1452-67. doi: 10.1084/jem.151.6.1452.
10
The involvement of suppressor T cells in Ir gene regulation of secondary antibody responses of primed (responder X nonresponder)F1 mice to macrophage-bound L-glutamic acid60-L-alanine30-L-tyrosine.抑制性T细胞参与致敏（反应者×无反应者）F1小鼠对巨噬细胞结合的L-谷氨酸60-L-丙氨酸30-L-酪氨酸二次抗体反应的Ir基因调控。
J Exp Med. 1978 Nov 1;148(5):1324-37. doi: 10.1084/jem.148.5.1324.
引用本文的文献
1
Hapten-specific T cell responses to 4-hydroxy-3-nitrophenyl acetyl. VIII. Suppressor cell pathways in cutaneous sensitivity responses.对半抗原4-羟基-3-硝基苯乙酰的特异性T细胞反应。VIII. 皮肤敏感性反应中的抑制细胞途径。
J Exp Med. 1981 Apr 1;153(4):811-22. doi: 10.1084/jem.153.4.811.
2
3
Suppressor T-cell mechanisms in contact sensitivity. III. Apparent non-major histocompatibility complex restriction is a result of multiple sets of major histocompatibility complex-specific suppressor T cells induced by syngeneic 2,4-dinitrophenyl-modified lymphoid cells.接触敏感性中的抑制性T细胞机制。III. 明显的非主要组织相容性复合体限制是由同基因2,4-二硝基苯基修饰的淋巴细胞诱导的多组主要组织相容性复合体特异性抑制性T细胞的结果。
J Exp Med. 1979 Sep 19;150(3):676-92. doi: 10.1084/jem.150.3.676.
4
Suppression of idiotype and generation of suppressor T cells with idiotype-conjugated thymocytes.用独特型结合的胸腺细胞抑制独特型并产生抑制性T细胞。
J Exp Med. 1979 Oct 1;150(4):909-18. doi: 10.1084/jem.150.4.909.
5
Hapten-specific T-cell responses to 4-hydroxy-3-nitrophenyl acetyl. I. Genetic control of delayed-type hypersensitivity by VH and I-A-region genes.对半抗原4-羟基-3-硝基苯乙酰的特异性T细胞应答。I. VH和I-A区基因对迟发型超敏反应的遗传控制。
J Exp Med. 1979 Jun 1;149(6):1336-48. doi: 10.1084/jem.149.6.1336.
6
Hapten-specific T-cell responses to 4-hydroxy-3-nitrophenyl acetyl. II. Demonstration of idiotypic determinants on suppressor T cells.对半抗原4-羟基-3-硝基苯乙酰的特异性T细胞应答。II. 抑制性T细胞上独特型决定簇的证明。
J Exp Med. 1979 Oct 1;150(4):761-76. doi: 10.1084/jem.150.4.761.
本文引用的文献
1
Paralysis to serum albumins in T and B lymphocytes in mice. Dose dependence, specificity and kinetics of escape.小鼠T淋巴细胞和B淋巴细胞中血清白蛋白麻痹。剂量依赖性、特异性及逃逸动力学
Eur J Immunol. 1974 Feb;4(2):120-5. doi: 10.1002/eji.1830040211.
2
Suppression of contact sensitivity by T cells in the mouse. I. Demonstration that suppressor cells act on the effector stage of contact sensitivity; and their induction following in vitro exposure to antigen.小鼠中T细胞对接触敏感性的抑制作用。I. 证明抑制细胞作用于接触敏感性的效应阶段；以及它们在体外暴露于抗原后的诱导产生。
Proc R Soc Lond B Biol Sci. 1974 Nov 5;187(1088):329-48. doi: 10.1098/rspb.1974.0078.
3
4
Genetic control of specific immune suppression. IV. Responsiveness to the random copolymer L-glutamic acid50-L-tyrosine50 induced in BALB/c mice by cyclophosphamide.特异性免疫抑制的遗传控制。IV. 环磷酰胺诱导BALB/c小鼠对随机共聚物L-谷氨酸50-L-酪氨酸50的反应性。
J Exp Med. 1976 Jul 1;144(1):277-81. doi: 10.1084/jem.144.1.277.
5
Control of t-lymphocyte and B-lymphocyte activation by two complementing Ir-GLphi immune response genes.两个互补的Ir-GLphi免疫反应基因对T淋巴细胞和B淋巴细胞激活的控制
J Exp Med. 1976 Apr 1;143(4):906-18. doi: 10.1084/jem.143.4.906.
6
Complementation of H-2-linked Ir genes in the mouse.小鼠中H-2连锁Ir基因的互补作用。
Proc Natl Acad Sci U S A. 1975 Sep;72(9):3671-5. doi: 10.1073/pnas.72.9.3671.
7
H-2 control of tolerance induction to L-glutamic acid, L-lysine-containing polymers.H-2对含L-谷氨酸、L-赖氨酸聚合物耐受性诱导的控制
J Immunol. 1978 Oct;121(4):1370-5.
8
Mechanisms of regulation of cell-mediated immune responses. I. Effect of the route of immunization with TNP-coupled syngeneic cells on the induction and suppression of contact sensitivity to picryl chloride.细胞介导免疫反应的调节机制。I. 用三硝基苯（TNP）偶联的同基因细胞免疫途径对氯化苦基接触敏感性诱导和抑制的影响。
J Immunol. 1978 May;120(5):1604-11.
9
Presence of common idiotypes on antibodies induced by glutamic acid-lysine-containing terpolymers in responder and nonresponder mice with the Ig-1b heavy chain allotype.在具有Ig-1b重链同种异型的应答和不应答小鼠中，由含谷氨酸-赖氨酸的三元共聚物诱导产生的抗体上存在共同独特型。
Eur J Immunol. 1977 Dec;7(12):865-71. doi: 10.1002/eji.1830071209.
10
Regulation of antibody heterogeneity by suppressor T cells: diminishing suppressor T cell activity increases the number of dinitrophenyl clones in mice immunized with dinitrophenyl-poly(Glu,Lys,Phe) or dinitrophenyl-poly(Glu,Lys,Ala).抑制性T细胞对抗体异质性的调节：降低抑制性T细胞活性会增加用二硝基苯基-聚（谷氨酸、赖氨酸、苯丙氨酸）或二硝基苯基-聚（谷氨酸、赖氨酸、丙氨酸）免疫的小鼠中二硝基苯基克隆的数量。
Proc Natl Acad Sci U S A. 1978 Jun;75(6):2914-7. doi: 10.1073/pnas.75.6.2914.
Zotero 插件