Meng F-W, Gao Z-L, Li L, Jie L-L, Yang P-F, Liang Z, Gao Y-W, Liu W-H
Department of Anatomy and Physiology, Shandong College of Traditional Chinese Medicine, Yantai, China.
Biological Science and Technology Institute, Weifang Medical University, Weifang, China.
Folia Morphol (Warsz). 2020;79(1):98-104. doi: 10.5603/FM.a2019.0044. Epub 2019 Apr 17.
The aim of the study was to investigate the regulatory mechanism of local lymphatic reconstruction after cupping therapy in a mouse model.
The lymphatic reconstruction process in the mouse tail after cupping therapy as well as the expression levels of the vascular endothelial identification molecule CD34, prospero homeobox protein 1 (PROX1), and lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) were investigated for a duration of 4 days through immunohistochemistry experiments.
On day 1 after cupping therapy, the CD34+ and LYVE-1+ cell densities were significantly increased, and the formed CD34+LYVE-1+ tubular structure started to express PROX1. This was followed by a decrease in both the CD34+ and LYVE-1+ stem cell densities to basal levels on the second day after cupping therapy. Both the CD34+ and LYVE-1+ cell densities subsequently increased again on the third day after cupping therapy. The increase in the LYVE-1+ density was accompanied by tubular structure formation, which is characteristic of lymphangiogenesis. In addition, the colocalisation of CD34+ and LYVE-1+ cells by immunohistochemistry suggests that the CD34+ stem cells differentiated into new lymphatic endothelial cells.
Our findings indicate that the mechanism underlying the therapeutic effect of cupping therapy involves upregulation of vascular and lymphatic endothelial markers (CD34+, LYVE-1+, and CD34+LYVE-1+) in local tissues, which in turn promotes local new lymphatic vessel formation through the expression of PROX1.
本研究旨在探讨拔罐疗法后小鼠模型局部淋巴重建的调控机制。
通过免疫组化实验，对拔罐疗法后小鼠尾巴的淋巴重建过程以及血管内皮识别分子CD34、prospero同源盒蛋白1（PROX1）和淋巴管内皮透明质酸受体1（LYVE-1）的表达水平进行了为期4天的研究。
拔罐疗法后第1天，CD34+和LYVE-1+细胞密度显著增加，形成的CD34+LYVE-1+管状结构开始表达PROX1。随后，拔罐疗法后第2天，CD34+和LYVE-1+干细胞密度均降至基础水平。拔罐疗法后第3天，CD34+和LYVE-1+细胞密度再次增加。LYVE-1+密度的增加伴随着管状结构的形成，这是淋巴管生成的特征。此外，免疫组化显示CD34+和LYVE-1+细胞共定位，提示CD34+干细胞分化为新的淋巴管内皮细胞。
我们的研究结果表明，拔罐疗法治疗效果的潜在机制涉及局部组织中血管和淋巴管内皮标志物（CD34+、LYVE-1+和CD34+LYVE-1+）的上调，进而通过PROX1的表达促进局部新淋巴管的形成。
