College of Chemistry and Molecular Sciences, Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education) , Wuhan University , Wuhan 430072 , China.
Anal Chem. 2019 May 21;91(10):6555-6560. doi: 10.1021/acs.analchem.8b05982. Epub 2019 Apr 29.
Different kinds of artificial drug delivery systems (DDS) have been widely exploited and utilized toward effective tumor therapy. Establishing a biocompatible DDS with a flexible release of the therapeutic agents has been a challenges and an impetus to the development of tumor diagnosis and therapy fields. Herein, the chemotherapeutic agents doxorubicin hydrochloride (DOX) and photosensitizer indocyanine green (ICG) were simultaneously packaged into the cavity of microvesicles (MVs) through the electroporation technique. With the aid of MVs-based DDS, the packaged therapeutic agents could be effectively delivered into the target tumor cells. The ambient temperature sharply increased because of controllable external near-infrared (NIR) laser irradiation, which induced the cracking of MVs-based DDS, directly accompanied by the dynamic and controllable release of DOX and ICG. Almost all the tumor cells could be killed by the synergistic effect of the released DOX and ICG. This research successfully established a smart DDS with NIR laser inducing controllable release of therapeutic agents for effective synergistic photochemotherapy toward cervical cancer.
不同类型的人工药物传递系统（DDS）已被广泛开发和利用，以实现有效的肿瘤治疗。建立具有治疗剂灵活释放的生物相容性 DDS 一直是肿瘤诊断和治疗领域发展的挑战和动力。在此，通过电穿孔技术将化疗药物盐酸多柔比星（DOX）和光增敏剂吲哚菁绿（ICG）同时包装到微泡（MV）的腔中。借助基于 MV 的 DDS，封装的治疗剂可以有效地递送到靶肿瘤细胞。由于可控的外部近红外（NIR）激光照射，环境温度急剧升高，这导致基于 MV 的 DDS 破裂，直接伴随着 DOX 和 ICG 的动态和可控释放。释放的 DOX 和 ICG 的协同作用几乎可以杀死所有的肿瘤细胞。这项研究成功地建立了一种智能 DDS，它可以在 NIR 激光诱导下控制释放治疗剂，用于宫颈癌的有效协同光化疗。
