直接从血清中估计高密度脂蛋白介导的胆固醇流出能力。
Direct Estimation of HDL-Mediated Cholesterol Efflux Capacity from Serum.
机构信息
Computational Medicine, Faculty of Medicine, University of Oulu and Biocenter Oulu, Oulu, Finland.
NMR Metabolomics Laboratory, School of Pharmacy, University of Eastern Finland, Kuopio, Finland.
出版信息
Clin Chem. 2019 Aug;65(8):1042-1050. doi: 10.1373/clinchem.2018.299222. Epub 2019 Apr 17.
BACKGROUND
HDL-mediated cholesterol efflux capacity (HDL-CEC) is a functional attribute that may have a protective role in atherogenesis. However, the estimation of HDL-CEC is based on in vitro cell assays that are laborious and hamper large-scale phenotyping.
METHODS
Here, we present a cost-effective high-throughput nuclear magnetic resonance (NMR) spectroscopy method to estimate HDL-CEC directly from serum. We applied the new method in a population-based study of 7603 individuals including 574 who developed incident coronary heart disease (CHD) during 15 years of follow-up, making this the largest quantitative study for HDL-CEC.
RESULTS
As estimated by NMR-spectroscopy, a 1-SD higher HDL-CEC was associated with a lower risk of incident CHD (hazards ratio, 0.86; 95%CI, 0.79-0.93, adjusted for traditional risk factors and HDL-C). These findings are consistent with published associations based on in vitro cell assays.
CONCLUSIONS
These corroborative large-scale findings provide further support for a potential protective role of HDL-CEC in CHD and substantiate this new method and its future applications.
背景
高密度脂蛋白（HDL）介导的胆固醇外排能力（HDL-CEC）是一种功能属性，可能在动脉粥样硬化形成中具有保护作用。然而，HDL-CEC 的估计基于体外细胞检测，这些检测既繁琐又妨碍了大规模表型分析。
方法
在这里，我们提出了一种经济高效的高通量核磁共振（NMR）光谱法，可直接从血清中估计 HDL-CEC。我们将新方法应用于一项基于人群的研究，该研究纳入了 7603 名个体，其中 574 名在 15 年的随访中发生了冠心病（CHD）。这是对 HDL-CEC 进行的最大规模的定量研究。
结果
通过 NMR 光谱法估计，HDL-CEC 每增加 1 个标准差，冠心病的发病风险就会降低（风险比为 0.86；95%CI，0.79-0.93，经传统危险因素和 HDL-C 校正）。这些发现与基于体外细胞检测的已发表关联一致。
结论
这些大规模的相互印证的发现为 HDL-CEC 在冠心病中的潜在保护作用提供了进一步的支持，并证实了这种新方法及其未来的应用。
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