参与外膜成分改变的基因的诱变。
Mutagenesis of the gene involved in alteration of outer membrane composition.
作者信息
Rahpeyma Sayyed Shahryar, Raheb Jamshid
机构信息
Department of Molecular Medicine, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
出版信息
Iran J Microbiol. 2019 Feb;11(1):67-74.
BACKGROUND AND OBJECTIVES
is a bacterial sigma factor of RNA polymerase which is involved in the expression of the genes which control regulons and play a critical role in survival against stresses. Few suitable vectors are available which could be maintained successfully in cells and could in particular be used as a suicide vector to make mutation in the gene. The aim of this study was to investigate if mutagenesis has impact on bacterial morphology in addition to cell division.
MATERIALS AND METHODS
A 0.603 kb BamHI-PstI fragment subclone of pICRPOS38Ω was cloned into linearized pLYLO3. The final construct, pLRPOS38 suicide vector, was introduced into . Then the cytoplasm of mutant strain and wild-type were investigated by transmission electron microscopy.
RESULTS
After successful subcloning of suicide vector into , based on TEM study, it was demonstrated that mutation in gene leads to decomposition of outer membrane of .
CONCLUSION
A suitable vector to make suicide mutation in was constructed for .
背景与目的
是RNA聚合酶的一种细菌σ因子，参与控制调节子的基因表达，并在抵抗压力的生存中发挥关键作用。很少有合适的载体能够在 细胞中成功维持，特别是可以用作自杀载体在 基因中产生突变。本研究的目的是调查 诱变除了对细胞分裂有影响外，是否还会对细菌形态产生影响。
材料与方法
将pICRPOS38Ω的一个0.603 kb BamHI-PstI片段亚克隆克隆到线性化的pLYLO3中。最终构建体pLRPOS38自杀载体被导入 。然后通过透射电子显微镜研究突变菌株和野生型的细胞质。
结果
在将自杀载体成功亚克隆到 后，基于透射电镜研究表明， 基因的突变导致 的外膜分解。
结论
构建了一种适合在 中进行自杀突变的载体。
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