肿瘤促进剂十四酰佛波醇乙酯使小鼠表皮鸟氨酸脱羧酶活性增加，这涉及到酶蛋白和信使核糖核酸含量的增加。

Increased mouse epidermal ornithine decarboxylase activity by the tumour promoter 12-O-tetradecanoylphorbol 13-acetate involves increased amounts of both enzyme protein and messenger RNA.

作者信息

Verma A K, Erickson D, Dolnick B J

出版信息

Biochem J. 1986 Jul 1;237(1):297-300. doi: 10.1042/bj2370297.

DOI:10.1042/bj2370297

PMID:3099748

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1146982/

Abstract

Evidence was sought that the tumour promoter 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced mouse epidermal ornithine decarboxylase (ODC, EC 4.1.1.17) activity involves both increased ODC mRNA and ODC protein. Application of 10 nmol of TPA to mouse skin led to a dramatic increase in soluble epidermal ODC activity which paralleled an increase in amount of enzymically active ODC protein as determined by gel electrophoresis of immunoprecipitated difluoromethyl[3H]ornithine-bound ODC. Application of TPA to mouse skin also resulted in an increase in ODC mRNA measured by dot-blot analysis using a radiolabelled cDNA probe. ODC mRNA induction preceded the increase in ODC activity by TPA. TPA-increased ODC mRNA displayed a single major band of 2.1 kilobases in size identified by the Northern blotting procedure.

摘要

研究证据表明，肿瘤促进剂12 - O - 十四烷酰佛波醇13 - 乙酸酯（TPA）诱导的小鼠表皮鸟氨酸脱羧酶（ODC，EC 4.1.1.17）活性涉及ODC mRNA和ODC蛋白的增加。将10 nmol的TPA应用于小鼠皮肤，导致可溶性表皮ODC活性急剧增加，这与通过免疫沉淀的二氟甲基[3H]鸟氨酸结合的ODC进行凝胶电泳测定的具有酶活性的ODC蛋白量的增加平行。将TPA应用于小鼠皮肤还导致使用放射性标记的cDNA探针通过斑点印迹分析测量的ODC mRNA增加。TPA诱导的ODC mRNA增加先于TPA诱导的ODC活性增加。通过Northern印迹法鉴定，TPA增加的ODC mRNA显示出一条大小为2.1千碱基的主要条带。