Matuda S, Nakano K, Saheki T
Biochem Int. 1986 Oct;13(4):599-608.
Pyruvate dehydrogenase complex and lipoamide dehydrogenase were purified from muscle of Ascaris lumbricoides var. suum which contains relatively a large amount of the complex. Molecular weights of three constituent enzymes of Ascaris pyruvate dehydrogenase complex were as follows; alpha- and beta-subunits of pyruvate dehydrogenase were 42,000 and 37,000, respectively, lipoate acetyltransferase was 76,000 and lipoamide dehydrogenase was 56,000. Furthermore, two unknown polypeptides having molecular weight of 46,000 and 41,000 were detected. Anti-Ascaris lipoamide dehydrogenase antibody precipitated three constituent enzymes and two unknown polypeptides, suggesting that lipoamide dehydrogenase not only binds tightly to complex, but also two unknown polypeptides bind tightly to complex.
丙酮酸脱氢酶复合体和硫辛酰胺脱氢酶是从猪蛔虫（Ascaris lumbricoides var. suum）肌肉中纯化得到的，猪蛔虫肌肉中含有相对大量的该复合体。猪蛔虫丙酮酸脱氢酶复合体的三种组成酶的分子量如下：丙酮酸脱氢酶的α亚基和β亚基分别为42,000和37,000，硫辛酸乙酰转移酶为76,000，硫辛酰胺脱氢酶为56,000。此外，还检测到两种分子量分别为46,000和41,000的未知多肽。抗猪蛔虫硫辛酰胺脱氢酶抗体沉淀出三种组成酶和两种未知多肽，这表明硫辛酰胺脱氢酶不仅与复合体紧密结合，而且两种未知多肽也与复合体紧密结合。
