锰离子和钒化合物对激素及福斯高林诱导的幼鼠卵巢腺苷酸环化酶刺激作用的影响
Action of Mn2+ and vanadium compounds on hormone and forskolin induced stimulation of juvenile rat ovarian adenylate cyclase.
作者信息
Tertrin-Clary C, Roy M, de la Llosa P
出版信息
Biochem Int. 1986 Dec;13(6):1019-35.
The effect of forskolin on the hormonal (LH, FSH) activation and on the stimulation provided by other effectors (Gpp(NH)p,NaF) of the juvenile rat ovarian adenylate cyclase was investigated. Forskolin exhibited a synergistic action with LH, FSH and Gpp(NH)p but not with NaF. Addition of Ca2+ was inhibitory over a concentration range from 10(-5) to 10(-2) M whereas EGTA enhanced the response at 5.10(-5) M and inhibited it at higher concentration. The cAMP production was increased by addition of Mn2+ at low concentration (up to 5 mM) but markedly decreased at higher concentration (30 mM). FSH induced cAMP production was completely abolished at 30 mM Mn2+. The effect of vanadyl ion was very similar to that of Mn2+ Vanadate anion on the contrary was without effect on FSH stimulation.
研究了福斯高林对幼鼠卵巢腺苷酸环化酶的激素（促黄体生成素、促卵泡生成素）激活作用以及其他效应物（鸟苷 5'-O-(3-硫代三磷酸)、氟化钠）刺激作用的影响。福斯高林与促黄体生成素、促卵泡生成素和鸟苷 5'-O-(3-硫代三磷酸)表现出协同作用，但与氟化钠无协同作用。在10⁻⁵至10⁻² M的浓度范围内添加钙离子具有抑制作用，而乙二醇双（2-氨基乙基醚）四乙酸在5×10⁻⁵ M时增强反应，在更高浓度时抑制反应。低浓度（高达5 mM）添加锰离子可增加环磷酸腺苷的产生，但在较高浓度（30 mM）时显著降低。在30 mM锰离子时，促卵泡生成素诱导的环磷酸腺苷产生完全被消除。钒离子的作用与锰离子非常相似，相反，钒酸根阴离子对促卵泡生成素刺激没有影响。
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