[急性髓系白血病患者中PD-L1和微小RNA-138-5p的表达及临床意义]
[Expression and Clinical Significance of PD-L1 and MicroRNA-138-5p in Patients with Acute Myeloid Leukemia].
作者信息
Huang Chun-Yan, Zha Xian-Feng, Wen Wang-Rong
机构信息
Center of Clinical Laboratorial Medicine，The First Affiliated Hospital of Jinan University，Guangzhou 510630, Guangdong Province, China.
Center of Clinical Laboratorial Medicine，The First Affiliated Hospital of Jinan University，Guangzhou 510630, Guangdong Province, China,E-mail:
出版信息
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2019 Apr;27(2):373-378. doi: 10.19746/j.cnki.issn.1009-2137.2019.02.010.
OBJECTIVE
To investigate the expression and clinical significance of PD-L1 and microRNA-138-5p in the peripheral blood mononuclear cells of patients with acute myeloid leukemia.
METHODS
The SYBR GreenⅠreal-time PCR was used to detect the expression levels of PD-L1 mRNA and miR-138 in 20 cases of primary AML, 9 cases of relapsed/refractory AML and 8 cases of complete remission. The samples of peripheral blood of 20 healthy peoples were used as controls.
RESULTS
The expression levels of PD-L1 in both the primary AML and the relapsed/refractory AML groups were significantly higher than those in the healthy control group (P<0.01), and the expression level of PD-L1 in the relapsed/refractory AML group was significantly higher than that in the primary AML group (P<0.01). The expression level of miR-138 in both the primary AML and the relapsed/refractory AML groups were significantly lower than that in the healthy control group (P<0.01). The 8 sampes out of 20 cases of primary AML patients achieved complete remission (CR) were collected and detected. The results showed that the expression level of miR-138 in the complete remission group was higher than that in the primary AML group (P<0.05), but the expression level of PD-L1 mRNA in the CR group was not significantly different from that in the primary AML group (P>0.05). and there was a negative correlation between PD-L1 mRNA and miR-138 in primary AML patients (P<0.05).
CONCLUSION
The expression of PD-L1 increases and the expression of miR-138 down-regulates in PBMNCs of AML patients, furthermore, both correlate each other.
目的
探讨程序性死亡配体1（PD-L1）和微小RNA-138-5p（miR-138-5p）在急性髓系白血病患者外周血单个核细胞中的表达及临床意义。
方法
采用SYBR GreenⅠ实时荧光定量聚合酶链反应（PCR）检测20例初治急性髓系白血病（AML）患者、9例复发/难治性AML患者及8例完全缓解患者外周血单个核细胞中PD-L1 mRNA和miR-138的表达水平。选取20例健康人外周血样本作为对照。
结果
初治AML组和复发/难治性AML组PD-L1表达水平均显著高于健康对照组（P<0.01），且复发/难治性AML组PD-L1表达水平显著高于初治AML组（P<0.01）。初治AML组和复发/难治性AML组miR-138表达水平均显著低于健康对照组（P<0.01）。收集20例初治AML患者中8例达到完全缓解（CR）的样本进行检测，结果显示CR组miR-138表达水平高于初治AML组（P<0.05），但CR组PD-L1 mRNA表达水平与初治AML组比较差异无统计学意义（P>0.05）。初治AML患者中PD-L1 mRNA与miR-138呈负相关（P<0.05）。
结论
AML患者外周血单个核细胞中PD-L1表达升高，miR-138表达下调，且二者相互关联。
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