Vergnolle Inès, Rieu Jean-Baptiste, Avet-Loiseau Hervé, Corre Jill, Vergez François
IUC-T laboratoire d'hématologie, CHU de Toulouse, Toulouse, France.
Ann Biol Clin (Paris). 2019 Apr 1;77(2):197-217. doi: 10.1684/abc.2019.1426.
Flow cytometric immunophenotyping has become essential for management of multiple myeloma (assessment of clonality, prognostic information on the risk of progression in gammopathy of undetermined significance, minimal residual disease monitoring). Immunophenotyping of bone marrow plasma cells is routinely used in the haematology laboratory of the University Hospital of Toulouse. To guarantee the reliability of this technique, the laboratory decided to check this method in compliance with the NF ISO EN 15189, standard for medical laboratories requirements. As expected, the method showed good technical performances. However, this initiative has demonstrated the importance of the sample quality and of the control of the preanalytical and analytical conditions. This process led us to maximize our professional pratices.
流式细胞术免疫表型分析已成为多发性骨髓瘤管理的关键（评估克隆性、意义未明的丙种球蛋白病进展风险的预后信息、微小残留病监测）。骨髓浆细胞的免疫表型分析在图卢兹大学医院的血液学实验室中常规使用。为确保该技术的可靠性，实验室决定按照医学实验室要求标准NF ISO EN 15189来检查此方法。不出所料，该方法显示出良好的技术性能。然而，这一举措证明了样本质量以及分析前和分析条件控制的重要性。这一过程促使我们优化专业操作。
