Awang-Kechik Noor-Hidayah, Ahmad Rohana, Doustjalali Saeid-Reza, Sabet Negar-Shafiei, Abd-Rahman Aida-Nur-Ashikin
Postgraduate, Centre for Paediatric Dentistry & Orthodontics Studies, Faculty of Dentistry, Universiti Teknologi MARA (UiTM), Malaysia.
Associate Professor, Faculty of Dentistry, Universiti Teknologi MARA, and Integrative Pharmacogenomics Institute, Puncak Alam Campus, Universiti Teknologi MARA, Malaysia.
J Clin Exp Dent. 2019 Mar 1;11(3):e269-e274. doi: 10.4317/jced.55546. eCollection 2019 Mar.
The biological responses involved during retention phase have been studied for many years but little is known about the effect of saliva proteome during retention phase of post-orthodontic treatment. This study aims to identify the protein profiles during retention phase in relation to biological processes involved by Liquid Chromatography Mass Spectrometry (LC-MS) approach.
A total of 5 ml of unstimulated saliva was collected from each subject (10 non-orthodontic patients and 15 post-orthodontic patients with 6-months retention phase). Samples were then subjected to LC-MS analysis. The expressed proteins were identified and compared between groups. Incisor irregularity for both maxilla and mandible were determined with Little's Irregularity Index at 6-months retention phase.
146 proteins and 135 proteins were expressed in control and 6-months retention phase group respectively. 15 proteins were identified to be co-expressed between groups. Immune system process was only detected in 6-months retention phase group. Detected protein in immune system process was identified as Tyrosine-protein kinase Tec. Statistical significant of incisor irregularity was only found in mandible at 6-months retention phase.
Our study suggests that immune system process protein which is Tyrosine-protein kinase Tec could be used as biomarker for prediction of stability during retention phase of post-orthodontic treatment. Orthodontics, proteomics, retention, LC-MS, saliva.
多年来一直在研究保持阶段所涉及的生物学反应，但对于正畸治疗后保持阶段唾液蛋白质组的影响知之甚少。本研究旨在通过液相色谱质谱（LC-MS）方法确定保持阶段与所涉及生物学过程相关的蛋白质谱。
从每位受试者（10名非正畸患者和15名处于6个月保持阶段的正畸后患者）收集5ml未刺激唾液。然后对样本进行LC-MS分析。鉴定并比较两组之间表达的蛋白质。在6个月保持阶段，用利特尔不规则指数确定上颌和下颌的切牙不规则度。
对照组和6个月保持阶段组分别表达了146种蛋白质和135种蛋白质。两组之间共鉴定出15种共同表达的蛋白质。免疫系统过程仅在6个月保持阶段组中检测到。在免疫系统过程中检测到的蛋白质被鉴定为酪氨酸蛋白激酶Tec。仅在6个月保持阶段的下颌中发现切牙不规则度具有统计学意义。
我们的研究表明，作为酪氨酸蛋白激酶Tec的免疫系统过程蛋白可作为正畸治疗后保持阶段稳定性预测的生物标志物。正畸学、蛋白质组学、保持、LC-MS、唾液。
