Department of Biology, Istanbul University, 34134 Suleymaniye, Istanbul, Turkey.
Department of Chemical and Biological Engineering, Koc University, Rumelifeneri Yolu, Sariyer, Istanbul, Turkey.
Funct Integr Genomics. 2019 Sep;19(5):715-727. doi: 10.1007/s10142-019-00675-2. Epub 2019 Apr 18.
Drought stress is one of the main environmental factors that affects growth and productivity of crop plants, including lentil. To gain insights into the genome-wide transcriptional regulation in lentil root and leaf under short- and long-term drought conditions, we performed RNA-seq on a drought-sensitive lentil cultivar (Lens culinaris Medik. cv. Sultan). After establishing drought conditions, lentil samples were subjected to de novo RNA-seq-based transcriptome analysis. The 207,076 gene transcripts were successfully constructed by de novo assembly from the sequences obtained from root, leaf, and stems. Differentially expressed gene (DEG) analysis on these transcripts indicated that period of drought stress had a greater impact on the transcriptional regulation in lentil root. The numbers of DEGs were 2915 under short-term drought stress while the numbers of DEGs were increased to 18,327 under long-term drought stress condition in the root. Further, Gene Ontology analysis revealed that the following biological processes were differentially regulated in response to long-term drought stress: protein phosphorylation, embryo development seed dormancy, DNA replication, and maintenance of root meristem identity. Additionally, DEGs, which play a role in circadian rhythm and photoreception, were downregulated suggesting that drought stress has a negative effect on the internal oscillators which may have detrimental consequences on plant growth and survival. Collectively, this study provides a detailed comparative transcriptome response of drought-sensitive lentil strain under short- and long-term drought conditions in root and leaf. Our finding suggests that not only the regulation of genes in leaves is important but also genes regulated in roots are important and need to be considered for improving drought tolerance in lentil.
干旱胁迫是影响包括兵豆在内的作物生长和生产力的主要环境因素之一。为了深入了解短期和长期干旱条件下兵豆根和叶的全基因组转录调控，我们对一个干旱敏感型兵豆品种（Lens culinaris Medik. cv. Sultan）进行了 RNA-seq 分析。在建立干旱条件后，对兵豆样本进行了基于 de novo RNA-seq 的转录组分析。通过从头组装从根、叶和茎获得的序列，成功构建了 207,076 个基因转录本。对这些转录本进行差异表达基因（DEG）分析表明，干旱胁迫期对兵豆根的转录调控影响更大。短期干旱胁迫下 DEG 的数量为 2915 个，而长期干旱胁迫条件下 DEG 的数量增加到 18327 个。此外，GO 分析表明，长期干旱胁迫下以下生物学过程受到差异调控：蛋白质磷酸化、胚胎发育、种子休眠、DNA 复制和根分生组织身份的维持。此外，参与昼夜节律和光受体的 DEGs 下调表明，干旱胁迫对内部振荡器有负面影响，这可能对植物的生长和生存产生不利影响。总的来说，本研究提供了一个详细的比较转录组反应，即在根和叶中，干旱敏感型兵豆品种在短期和长期干旱条件下的反应。我们的研究结果表明，不仅叶片中基因的调控很重要，而且根部调控的基因也很重要，需要考虑提高兵豆的耐旱性。
