College of Forestry and Prataculture, Ningxia University, Yinchuan, Ningxia, China.
Breeding Base for State Key Laboratory of Land Degradation and Ecological Restoration of North-Western China, Ningxia University, Yinchuan, Ningxia, China.
PeerJ. 2023 Jun 14;11:e15440. doi: 10.7717/peerj.15440. eCollection 2023.
is a shrub with high quality protein that has been widely utilized for forage grass in the semi-arid regions of China. This study aimed to enrich the currently available knowledge and clarify the detailed drought stress regulatory mechanisms in , and provide a theoretical foundation for the cultivation and resistance breeding of forage crops.
This study evaluates the response mechanism to drought stress by exploiting multiple parameters and transcriptomic analyses of a 1-year-old seedlings of in a pot experiment.
Drought stress significantly caused physiological changes in . The antioxidant enzyme activities and osmoregulation substance content of showed an increase under drought. Moreover, 3,978 and 6,923 differentially expressed genes were approved by transcriptome in leaves and roots. The transcription factors, hormone signal transduction, carbohydrate metabolism of regulatory network were observed to have increased. In both tissues, genes related to plant hormone signaling transduction pathway might play a more pivotal role in drought tolerance. Transcription factors families like basic helix-loop-helix (bHLH), vian myeloblastosis viral oncogene homolog (MYB), basic leucine zipper (bZIP) and the metabolic pathway related-genes like serine/threonine-phosphatase 2C (PP2C), SNF1-related protein kinase 2 (SnRK2), indole-3-acetic acid (IAA), auxin (AUX28), small auxin up-regulated rna (SAUR), sucrose synthase (SUS), sucrosecarriers (SUC) were highlighted for future research about drought stress resistance in .
Our study posited mainly participate in various physiological and metabolic activities to response severe drought stress, by regulating the expression of the related genes in hormone signal transduction. These findings, which may be valuable for drought resistance breeding, and to clarify the drought stress regulatory mechanisms of and other plants.
是一种具有高质量蛋白质的灌木，已在中国半干旱地区广泛用作饲料草。本研究旨在丰富目前的知识，阐明的详细干旱胁迫调控机制，并为饲料作物的栽培和抗性育种提供理论基础。
本研究通过对盆栽 1 年生幼苗进行多种参数评价和转录组分析，评估其对干旱胁迫的响应机制。
干旱胁迫显著影响了的生理变化。干旱胁迫下，抗氧化酶活性和渗透调节物质含量增加。此外，在叶片和根中分别有 3978 个和 6923 个差异表达基因通过转录组得到验证。观察到调控网络中的转录因子、激素信号转导、碳水化合物代谢增加。在这两种组织中，与植物激素信号转导途径相关的基因可能在耐旱性中发挥更关键的作用。转录因子家族如碱性螺旋-环-螺旋（bHLH）、髓样白血病病毒癌基因同源物（MYB）、碱性亮氨酸拉链（bZIP）以及与代谢途径相关的基因如丝氨酸/苏氨酸磷酸酶 2C（PP2C）、SNF1 相关蛋白激酶 2（SnRK2）、吲哚-3-乙酸（IAA）、生长素（AUX28）、小生长素上调 RNA（SAUR）、蔗糖合酶（SUS）、蔗糖载体（SUC）在干旱胁迫抗性的研究中备受关注。
本研究认为主要通过调节激素信号转导相关基因的表达，参与各种生理和代谢活动来应对严重干旱胁迫。这些发现可能对耐旱性育种有价值，并有助于阐明和其他植物的干旱胁迫调控机制。
