Cuervo-Arango Juan, Claes Anthony N, Beitsma Mabel, Stout Tom A E
Faculty of Veterinary Medicine, Department of Equine Sciences, Utrecht University, Utrecht, The Netherlands.
Faculty of Veterinary Medicine, Department of Equine Sciences, Utrecht University, Utrecht, The Netherlands.
J Equine Vet Sci. 2019 Apr;75:74-77. doi: 10.1016/j.jevs.2019.01.015. Epub 2019 Feb 6.
The in vitro production of embryos by ovum pickup (OPU) and intracytoplasmic sperm injection (ICSI) is gaining popularity among horse breeders and veterinarians. Various collection media are available for flushing follicles during OPU. The objective of this study was to determine whether the type of flushing media used to aspirate follicles and collect oocytes influences the outcome of a commercial equine OPU-ICSI program. Two commercial embryo flushing media (EFM1 and EFM2) supplemented with heparin were compared with a flushing media designed specifically for the collection of oocytes (oocyte flushing media [OFM]) on the outcome of OPU-ICSI parameters in 234 Warmblood mares. The OPU-ICSI performed in mares using one of the EFM1 resulted in a lower (P < .05) blastocyst rate and blastocysts per OPU-ICSI session (11.9 ± 13.2%, 0.88 ± 1.3) than the OFM (19.2 ± 15.2%, 1.24 ± 1.2). Unlike the EFM2 solution, the heparin used to prepare the EFM1 contained preservatives including benzyl alcohol, a component known to alter the oocyte membrane, which might have been responsible for the lower developmental competence of oocytes collected with EFM1. In conclusion, exposure of oocytes (<1.5 hours) to one of the flushing medium tested in this study affected negatively the outcome of the OPU-ICSI commercial program when compared with flushing media designed for collection of equine oocytes. Care should be taken when choosing the components of the flushing media used to collect oocytes. Further research should be carried out to confirm the potential negative effect of the preservatives used in multidose heparin vials.
通过采卵（OPU）和卵胞浆内单精子注射（ICSI）进行胚胎的体外生产在马匹饲养者和兽医中越来越受欢迎。在OPU过程中，有多种采集介质可用于冲洗卵泡。本研究的目的是确定用于抽吸卵泡和收集卵母细胞的冲洗介质类型是否会影响商业马OPU-ICSI程序的结果。将两种添加肝素的商业胚胎冲洗介质（EFM1和EFM2）与一种专门为收集卵母细胞设计的冲洗介质（卵母细胞冲洗介质[OFM]）在234匹温血母马的OPU-ICSI参数结果方面进行了比较。使用EFM1之一对母马进行的OPU-ICSI所产生的囊胚率和每次OPU-ICSI操作的囊胚数（11.9±13.2%，0.88±1.3）低于OFM（19.2±15.2%，1.24±1.2）（P<0.05）。与EFM2溶液不同，用于制备EFM1的肝素含有包括苯甲醇在内的防腐剂，苯甲醇是一种已知会改变卵母细胞膜的成分，这可能是导致用EFM1收集的卵母细胞发育能力较低的原因。总之，与专为收集马卵母细胞设计的冲洗介质相比，本研究中测试的一种冲洗介质使卵母细胞暴露（<1.5小时）对OPU-ICSI商业程序的结果产生了负面影响。在选择用于收集卵母细胞的冲洗介质成分时应谨慎。应进行进一步研究以确认多剂量肝素瓶中使用的防腐剂的潜在负面影响。
