Department of Orthopaedic Surgery, The No. 1 People's Hospital of Jingzhou, Jingzhou, China.
Eur Rev Med Pharmacol Sci. 2019 Apr;23(7):2744-2749. doi: 10.26355/eurrev_201904_17547.
Recently, long noncoding RNAs (lncRNAs) have got much attention for their role in tumor progression. LncRNA AFAP1-AS1 was studied in this research to identify how it affects the development and proliferation of osteosarcoma.
The mRNA expression of AFAP1-AS1 in osteosarcoma cells and tissue samples was detected by quantitative real-time polymerase chain reaction (qRT-PCR). Moreover, cell proliferation assay and Matrigel assay were performed. Furthermore, the underlying mechanism was explored by using qRT-PCR and Western blot assay.
The expression level of AFAP1-AS1 was higher in osteosarcoma samples than that in adjacent tissues. In addition, the proliferation and invasion were inhibited after AFAP1-AS1 was downregulated in vitro. Besides, the mRNA and protein expression levels of brain derived neurotrophic factor (BDNF) were reduced after downregulation of AFAP1-AS1. Furthermore, the expression level of BDNF was positively related to the expression of AFAP1-AS1 in osteosarcoma tissues.
AFAP1-AS1 could enhance the proliferation and invasion of osteosarcoma cells by upregulating BDNF, which might be a potential therapeutic target in osteosarcoma.
长链非编码 RNA（lncRNAs）在肿瘤进展中的作用受到了广泛关注。本研究旨在探讨 lncRNA AFAP1-AS1 如何影响骨肉瘤的发生发展和增殖。
采用实时定量聚合酶链反应（qRT-PCR）检测骨肉瘤细胞和组织样本中 AFAP1-AS1 的 mRNA 表达。此外，还进行了细胞增殖试验和 Matrigel 试验。进一步采用 qRT-PCR 和 Western blot 试验探讨其潜在机制。
骨肉瘤组织中 AFAP1-AS1 的表达水平高于相邻组织。此外，体外下调 AFAP1-AS1 后可抑制细胞增殖和侵袭。此外，下调 AFAP1-AS1 后脑源性神经营养因子（BDNF）的 mRNA 和蛋白表达水平降低。此外，骨肉瘤组织中 AFAP1-AS1 的表达与 BDNF 的表达呈正相关。
AFAP1-AS1 通过上调 BDNF 增强骨肉瘤细胞的增殖和侵袭能力，可能成为骨肉瘤潜在的治疗靶点。
