a Department of Maria Plus IVF center , Maria Plus Fertility Hospital , Songpa-gu , Seoul , Republic of Korea.
b Division of Biotechnology , College of Life Sciences and Biotechnology, Korea University , Seongbuk-gu , Seoul , Republic of Korea.
Syst Biol Reprod Med. 2019 Aug;65(4):333-341. doi: 10.1080/19396368.2019.1602681. Epub 2019 Apr 19.
maturation (IVM) has evolved as a clinical treatment option in assisted reproductive technology. However, the poor developmental potential of germinal vesicle (GV)-stage oocytes is still suboptimal. This study's objective was to evaluate the effect of a microvibration culture system (MVC) during IVM and/or culture (IVC) on the clinical outcomes and the embryonic development potential of human GV-stage oocytes collected from human chorionic gonadotropin (HCG)-primed IVM and fertilization-embryo transfer (IVM/F-ET) cycles of patients with polycystic ovaries (PCO). A total of 206 HCG-primed IVM/F-ET cycles were divided into four groups according to the microvibration and static culture system applied during IVM and/or IVC: Group SS (static system during both IVM and IVC); Group SV (static system during IVM alternated with microvibration system during IVC); Group VS (microvibration system during IVM alternated with static system during IVC), and Group VV (microvibration system during both IVM and IVC). The results indicate that the rates of MII oocytes per cycle, fertilization, and cleavage were not significantly different between the groups. The rate of good-quality embryos in Group SV tended to be higher than the rate in Groups SS and VS, but there was no significant difference between Group SS and Group SV. Clinical pregnancy, implantation, and live birth rates of Groups SV and VS were slightly higher than those of Group SS. However, the rate of good-quality embryos with at least six cells on day 4, the clinical pregnancy, implantation, and live births in Group VV were significantly higher than those in Group SS. These results indicate that, compared with the static culture system, the MVC system applied for both IVM and IVC seems to improve the clinical outcomes and the quality of embryos of GV oocytes derived from HCG-primed IVM/F-ET cycles in PCO patients. PCO: polycystic ovaries; HCG: human chorionic gonadotropin; GV: germinal vesicle; MII: metaphase II; IVM: maturation; IVF: fertilization; IVC: culture: MVC: microvibration culture; SC: static culture; ICSI: intracytoplasmic sperm injection; IVM/F-ET: IVM and fertilization-embryo transfer; AMH: anti-Mullerian hormone; OHSS: ovarian hyperstimulation syndrome.
卵母细胞体外成熟（IVM）已作为辅助生殖技术中的一种临床治疗选择而发展起来。然而，卵母细胞生发泡期（GV）的发育潜力仍然较差。本研究旨在评估微振动培养系统（MVC）在人绒毛膜促性腺激素（HCG）诱导的 IVM 和受精-胚胎移植（IVM/F-ET）周期中对多囊卵巢（PCO）患者 GV 期卵母细胞的临床结局和胚胎发育潜能的影响。总共 206 个 HCG 诱导的 IVM/F-ET 周期根据 IVM 和/或 IVC 期间应用的微振动和静态培养系统分为四组：SS 组（IVM 和 IVC 期间均为静态系统）；SV 组（IVM 期间为静态系统，IVC 期间为微振动系统）；VS 组（IVM 期间为微振动系统，IVC 期间为静态系统）和 VV 组（IVM 和 IVC 期间均为微振动系统）。结果表明，各组间每周期的 MII 卵母细胞数、受精率和卵裂率无显著差异。SV 组的优质胚胎率有高于 SS 组和 VS 组的趋势，但 SS 组和 SV 组之间无显著差异。SV 组和 VS 组的临床妊娠率、种植率和活产率略高于 SS 组。然而，VV 组第 4 天至少有 6 个细胞的优质胚胎率、临床妊娠率、种植率和活产率均显著高于 SS 组。这些结果表明，与静态培养系统相比，应用于 IVM 和 IVC 的 MVC 系统似乎可改善 PCO 患者 HCG 诱导的 IVM/F-ET 周期中 GV 卵母细胞的临床结局和胚胎质量。
