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[用于研究正常腹膜巨噬细胞与肿瘤细胞相互作用的扫描电子显微镜方法]

[Scanning electron microscopic method for studying the interaction of normal peritoneal macrophages with tumor cells].

作者信息

Rytenko A N, Balashov K E, Sergeev A V

出版信息

Eksp Onkol. 1986;8(6):52-7.

PMID:3100268
Abstract

It is shown that the damage action of normal peritoneal macrophages of mice includes inhibition of the proliferating activity of the mastocytoma P-815 cells and their complete destruction accompanied by the breaks of their cytoplasmic membrane. Scanning electron microscopy was used to reveal two types of contacts between peritoneal macrophages and cells P-815; the loss of microvilli and the appearance of blebs on cell surface in the case of adsorption of P-815 cells on macrophages; the contact through long filopodia which are sent forth by macrophages in the case when contacting cells are at some distance from each other.

摘要

结果表明,小鼠正常腹腔巨噬细胞的损伤作用包括抑制肥大细胞瘤P - 815细胞的增殖活性并使其完全破坏,同时伴有细胞质膜破裂。采用扫描电子显微镜揭示了腹腔巨噬细胞与P - 815细胞之间的两种接触类型:当P - 815细胞吸附在巨噬细胞上时,细胞表面微绒毛丧失并出现泡状突起;当接触细胞彼此相距一定距离时,巨噬细胞伸出长丝状伪足进行接触。

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