Department of Animal Medicine (Infectious Diseases), Faculty of Veterinary Medicine, Benha University, Egypt.
Department of Clinical Pathology, Faculty of Veterinary Medicine, Benha University, Egypt.
Acta Trop. 2019 Jul;195:1-5. doi: 10.1016/j.actatropica.2019.04.019. Epub 2019 Apr 16.
Johne's disease is chronic enteric incurable disease in ruminant, is caused by Mycobacterium avium subsp. paratuberculosis (MAP). The data about incidence and economic importance of the disease in Egypt are scare. The present study investigated the prevalence of Johne`s disease among cattle in some localities in Egypt. The results revealed that the infection was significantly higher in older animals than young animals (P = <0.05). The prevalence of the disease was higher in Gharbia governorate (19.6%) in comparison with other studied area. To determine the efficiency of diagnostic tests, 138 fecal samples were examined by culture and real-time PCR, the result showed slight agreement between both tests for detection of MAP in feces. Sequence analysis of IS1311 gene for isolated MAP strain from Egypt revealed high identity (98%) with other MAP strains from Germany and Netherland. MAP belongs to a subspecies genetically different from Mycobacterium avium subsp. Hominissuis (MAH) and Mycobacterium avium subsp. avium (MAA), therefore these results are as would be expected. This study confirms the circulation of MAP among cattle in Egypt and highlights the need for more study on the disease in the Middle East.
约翰氏病是反刍动物的慢性、无法治愈的肠道疾病，由分枝杆菌副结核亚种（MAP）引起。关于埃及该病的发病率和经济重要性的数据很少。本研究调查了埃及一些地区牛群中约翰氏病的流行情况。结果表明，感染在老年动物中明显高于年轻动物（P<0.05）。与其他研究区域相比，加比亚省（19.6%）的该病发病率更高。为了确定诊断试验的效率，对 138 份粪便样本进行了培养和实时 PCR 检测，结果表明这两种检测方法在检测粪便中的 MAP 方面具有轻微的一致性。对从埃及分离的 MAP 菌株的 IS1311 基因进行序列分析显示，与德国和荷兰的其他 MAP 菌株高度同源（98%）。MAP 属于与分枝杆菌副结核亚种 Hominissuis（MAH）和分枝杆菌副结核亚种 avium（MAA）在遗传上不同的亚种，因此这些结果是意料之中的。本研究证实了 MAP 在埃及牛群中的传播，并强调需要在中东地区对该疾病进行更多的研究。
