College of Life Science, Liaoning Normal University, Dalian, 116081, China; Lamprey Research Center, Liaoning Normal University, Dalian, 116081, China.
College of Life Science, Liaoning Normal University, Dalian, 116081, China; Lamprey Research Center, Liaoning Normal University, Dalian, 116081, China.
Fish Shellfish Immunol. 2019 Jul;90:446-455. doi: 10.1016/j.fsi.2019.04.045. Epub 2019 Apr 16.
Adiponectin (APN) is an important cytokine secreted by fat cells that is responsible for regulating numerous biological functions. However, the APN gene in lamprey and its precise function remain unidentified. In this study, the full-length cDNA sequence of L-APN was cloned, and it encoded a protein of 267 amino acid residues with a globular domain. The results of immunohistochemistry and FACS assays showed that APN protein was distributed in multiple tissues. L-APN expression in the supraneural body (SB) and leukocytes was differentially upregulated in response to Gram-negative bacteria, Gram-positive bacteria and poly (I:C). The expression levels of inflammatory cytokines were upregulated, and a proapoptotic effect was stimulated in SB cells treated with recombinant APN. Furthermore, L-APN could inhibit cell proliferation and arrest cell growth in the G1 phase. In summary, the APN protein from the lamprey plays an important role in inhibiting cell proliferation, inducing the production of inflammatory cytokines and promoting cell apoptosis, and it is also involved in immune responses and immune defenses. Our data provide insights into the evolutionary origin of the structure and function of APN gene.
脂联素（APN）是脂肪细胞分泌的一种重要细胞因子，负责调节多种生物学功能。然而，在七鳃鳗中，APN 基因及其确切功能仍未被确定。本研究克隆了全长的 L-APN cDNA 序列，其编码的蛋白含有 267 个氨基酸残基的球形结构域。免疫组织化学和流式细胞术检测结果表明，APN 蛋白分布于多种组织中。在对革兰氏阴性菌、革兰氏阳性菌和 poly（I:C）的反应中，脑上腺（SB）和白细胞中的 L-APN 表达水平呈差异上调。重组 APN 处理 SB 细胞后，炎症细胞因子的表达水平上调，并刺激细胞凋亡。此外，L-APN 可抑制细胞增殖并将细胞生长阻滞在 G1 期。综上所述，七鳃鳗的 APN 蛋白在抑制细胞增殖、诱导炎症细胞因子产生和促进细胞凋亡方面发挥重要作用，并且还参与免疫反应和免疫防御。我们的数据为 APN 基因的结构和功能的进化起源提供了新的见解。
