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兔精子上透明带结合位点的定位以及溶解的透明带诱导顶体反应

Localization of zona pellucida binding sites on rabbit spermatozoa and induction of the acrosome reaction by solubilized zonae.

作者信息

O'Rand M G, Fisher S J

出版信息

Dev Biol. 1987 Feb;119(2):551-9. doi: 10.1016/0012-1606(87)90058-3.

Abstract

The binding of mammalian spermatozoa to the egg's extracellular coat, the zona pellucida, is a complex process which culminates in species-specific penetration of the sperm to the egg plasma membrane. To investigate where on the spermatozoon's surface the zona binding sites are located, whole rabbit zonae were labeled with FITC, heat solubilized and used to observe the surface binding patterns on live spermatozoa. Before the acrosome reaction the zona binding sites are located either over the entire head as well as the middle piece or alternatively in patches along the apical ridge of the head. After the acrosome reaction there is a 29% loss of fluorescence and the zona binding sites are present in the posterior aspect of the acrosomal region, the anterior postacrosomal region and the middle piece. These results demonstrate the presence of zona binding sites after the acrosome reaction which would account for the sperm's ability to remain bound to the zona after the acrosome reaction. Further, we report for the first time that solubilized rabbit zonae pellucidae will induce the acrosome reaction in in vitro capacitated rabbit sperm whereas solubilized pig zonae pellucidae will not. Since rabbit sperm bind pig zonae, the induction and specificity of the physiological acrosome reaction must reside in the affinity of the binding rather than the binding itself.

摘要

哺乳动物精子与卵子的细胞外被——透明带的结合是一个复杂的过程,最终导致精子以物种特异性方式穿透至卵子质膜。为了研究精子表面的透明带结合位点位于何处,将完整的兔透明带用异硫氰酸荧光素(FITC)标记,加热使其溶解,然后用于观察活精子的表面结合模式。在顶体反应之前,透明带结合位点要么位于整个头部以及中段,要么沿着头部的顶嵴呈斑块状分布。顶体反应后,荧光损失29%,透明带结合位点存在于顶体区域的后部、顶体后前部区域和中段。这些结果表明顶体反应后存在透明带结合位点,这可以解释精子在顶体反应后仍能与透明带结合的能力。此外,我们首次报道,溶解的兔透明带会诱导体外获能的兔精子发生顶体反应,而溶解的猪透明带则不会。由于兔精子能结合猪透明带,生理性顶体反应的诱导和特异性必定在于结合的亲和力而非结合本身。

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