Krogenaes A, Andersen Berg K, Hafne A L, Engeland E
Department of Reproduction and Forensic Medicine, Norwegian College of Veterinary Medicine, Oslo.
Acta Vet Scand. 1994;35(1):17-26. doi: 10.1186/BF03548352.
Membrane alterations in bull spermatozoa after freezing and thawing and after the process of in vitro capacitation and fertilization were studied by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Even if the majority of the spermatozoa exhibited intact membranes after freezing and thawing (90%), one could distinguish between 3 types of membrane defects depending of the different structures involved. The first type showed loss of plasmalemma over the entire acrosome. In the second category the anterior part of the outer acrosomal membrane exhibited a pronounced extension, but was covered by a partly intact plasmalemma. The last category consisted of spermatozoa with extensive vesiculation and disruption of plasmalemma and the outer acrosomal membrane. This type of defect could not easily be distinguished from a true acrosome reaction. The cumulus cells showed an active phagocytosis of both intact and acrosome reacted spermatozoa.
通过扫描电子显微镜（SEM）和透射电子显微镜（TEM）研究了冻融后以及体外获能和受精过程中公牛精子的膜变化。即使大多数精子在冻融后膜完整（90%），根据涉及的不同结构，仍可区分出3种膜缺陷类型。第一种类型表现为整个顶体上质膜缺失。第二类中，顶体外膜前部有明显延伸，但被部分完整的质膜覆盖。最后一类由具有广泛囊泡化以及质膜和顶体外膜破裂的精子组成。这种缺陷类型不易与真正的顶体反应区分开来。卵丘细胞对完整和顶体反应的精子均表现出活跃的吞噬作用。
